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| NRTUAs感染对小鼠的组织荷虫量及脾脏细胞因子转录水平的影响 |
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任超1,2,王超越2,卢春霞2,王思2,段春慧2,刘贤勇2,索勋2*
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| (1.天津农学院 动物科学与动物医学学院/天津市农业动物繁育与健康养殖重点实验室, 天津 300384;2.中国农业大学 动物医学院, 北京100193) |
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| 摘要: |
| 为进一步研究非增殖型尿嘧啶营养缺陷型弓形虫(NRTUAs)对小鼠的致病性，本研究以NRTUAs和Δku80虫株分别感染小鼠，每日检测各组小鼠的组织荷虫量，并检测感染后1~3 d脾脏相关细胞因子的转录水平。结果表明:1)NRTUAs感染的小鼠不能在7 d内清除虫体，NRTUAs刺激小鼠脾脏上调IL-12、TNF-α和IL-10的表达，并诱导温和的炎症反应；2)Δku80虫株感染的小鼠在感染后6 d内死亡，组织荷虫量平均水平高于NRTUAs感染的小鼠，Δku80虫株刺激小鼠脾脏上调IL-12、TNF-α、IL-1β、IL-6、IFN-γ和IL-10的表达，诱导剧烈的炎症反应而导致小鼠死亡。综上，NRTUAs对小鼠的致病性较低，NRTUAs组织荷虫量平均水平低于Δku80组，诱导机体的炎症反应显著轻于Δku80组，为NRTUAs作为畜禽免疫增强剂的开发提供研究基础。 |
| 关键词: 非增殖型尿嘧啶营养缺陷型弓形虫 组织荷虫量 细胞因子 小鼠 致病性 |
| DOI：10.11841/j.issn.1007-4333.2022.12.15 |
| 投稿时间：2022-04-15 |
| 基金项目:国家自然科学基金项目(31902277) |
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| Impacts of NRTUAs infection on the parasite load and the transcriptional levels of splenic cytokines in mice |
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REN Chao1,2,WANG Chaoyue2,LU Chunxia2,WANG Si2,DUAN Chunhui2,LIU Xianyong2,SUO Xun2*
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| (1.Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry/College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;2.College of Veterinary Medicine, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| In order to further explore the pathogenicity of nonreplicating Toxoplasma uracil auxotrophs(NRTUAs)in mice, mice were infected with NRTUAs or Δku80 strain to detect parasite load for each group daily and the transcriptional levels of related splenic cytokines within 1 to 3 days after parasite infection. The results indicated that: 1)NRTUAs were not completely removed from the mice within 7 days. NRTUAs stimulated the spleen of mice to up-regulate the expression of IL-12, TNF-α and IL-10, which induced a mild inflammatory response. 2)While mice infected with Δku80 strain were dead within 6 days and the average parasite load was higher than that of NRTUAs infected mice. Δku80 strain stimulated the spleen of mice to up-regulate the expression of IL-12, TNF-α, IL-1β, IL-6, IFN-γ and IL-10, which induced severe inflammatory response to cause the death of mice. In summary, the pathogenicity of NRTUAs to mice was low and the average parasite load was less than that of Δku80 strain infected mice. The inflammatory response of mice stimulated by NRTUAs was lighter than that of Δku80 strain infected mice. This study laid a theoretical foundation for the application of NRTUAs as immunopotentiator in livestock and poultry breeding. |
| Key words: nonreplicating Toxoplasma uracil auxotrophs parasite load cytokines mice pathogenicity |
