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| 奶牛与肉牛不同生长发育阶段肝脏转录组比较分析 |
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周乐1,2,石彩霞1,2,常晨城1,2,王宇3,满达1,2,高静4,张文广1,2*
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| (1.内蒙古农业大学 动物科学学院, 呼和浩特 010018;2.内蒙古自治区农业基因组大数据工程研究中心, 呼和浩特 010018;3.内蒙古农业大学 兽医学院, 呼和浩特 010018;4.内蒙古农业大学 计算机与信息工程学院, 呼和浩特 010018) |
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| 摘要: |
| 为探究奶牛与肉牛肝脏组织在不同生长发育阶段中的差异，利用RNA-seq技术和生物信息学的研究方法，分别对海福特肉牛与荷斯坦奶牛6、9和12月龄3个不同生长发育阶段的肝脏组织进行基因差异表达及表达趋势分析。结果表明:1)海福特肉牛与荷斯坦奶牛之间在6、9和12月龄分别获得的差异表达基因为699、486和769个；2)对海福特肉牛与荷斯坦奶牛之间的差异表达基因进行功能注释，发现细胞色素P450信号通路和PPAR信号通路中差异表达基因的表达量在肉牛与奶牛之间存在差异，并且在不同生长阶段表达也不同。其中与脂肪细胞增殖和脂质代谢有关的功能基因:ACOX1、APOA1和ACADM，与天然免疫相关的通路中BoLA-DQB、BLA-DQB和BoLA-DQA 这3个基因在海福特肉牛中的表达均高于荷斯坦奶牛；3)STEM分析结果显示荷斯坦奶牛与海福特肉牛肝脏组织在生长发育阶段中具有相同表达趋势中，奶牛与肉牛的时序性表达基因与代谢途径相关信号通路上存在差异。综上，本研究表明与脂质代谢和脂肪生成等代谢过程有关的途径可能在协同调控奶牛与肉牛生长发育阶段中发挥的作用及表达模式存在差异，这为深入研究奶牛与肉牛在生长发育阶段中的比较提供了一定的基础数据。 |
| 关键词: 肝脏 奶牛 肉牛 短时序表达分析 生长发育 |
| DOI：10.11841/j.issn.1007-4333.2022.12.13 |
| 投稿时间：2022-01-07 |
| 基金项目:内蒙古自治区自然科学基金重大项目(2021ZD05)；内蒙古自治区科技重大专项课题(2021ZD0009)；内蒙古自治区重大专项(2019ZD016)；内蒙古自治区重点项目(2019GG363)；内蒙古自治区重大专项(2020ZD003)；内蒙古自治区科技计划(2021GG008) |
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| Comparative liver transcriptome analysis of dairy cow and beef cattle at different growth and development stages |
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ZHOU Le1,2,SHI Caixia1,2,CHANG Chencheng1,2,WANG Yu3,MAN Da1,2,GAO Jing4,ZHANG Wenguang1,2*
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| (1.College of Animal Science and Technology, Inner Mongolia Agricultural University, Hohhot 010018, China;2.Key Laboratory of Animal Genetics, Breeding and Reproduction of the Inner Mongolia Autonomous Region, College of Animal Science and Technology, Inner Mongolia Agricultural University, Hohhot 010018, China;3.Veterinary College, Inner Mongolia Agricultural University; Hohhot 010018, China;4.College of Computer and Information Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China) |
| Abstract: |
| In order to explore the differences of liver tissues at different growth and development stages between dairy cow and beef cattle, the differential expression and expression trend of liver tissues of dairy cattle and beef cattle at different growth and development stages of 6 months, 9 months and 12 months were studied by using RNA-Seq technology and bioinformatics methods. The results showed that: 1)About 699, 486 and 769 differentially expressed genes between Hereford and Holstein cattle were obtained at 6, 9 and 12 months of age, respectively; 2)The functional annotation of differentially expressed genes between Hereford and Holstein cattle showed that the expression levels of differentially expressed genes in cytochrome P450 signaling pathway and PPAR signaling pathway were different between beef and dairy cattle, and the expression levels were also different at different growth stages. Among them, the functional genes related to adipocyte proliferation and lipid metabolism, ACOX1, APOA1 and ACADM, BoLA-DQB, BLA-DQB and BoLA-DQA5 in the natural immunity-related pathways, were all expressed in Hereford higher than Holstein; 3)STEM analysis results showed that Holstein and Hereford cattle had the same expression trend in liver tissues during growth and development stage, and there were differences in the sequential expression genes and metabolic pathway related signaling pathways between beef and dairy cattle. In conclusion, this study shows that pathways related to lipid metabolism and lipogenesis may have different roles and expression patterns in synergistically regulating the growth and development of dairy and beef cattle. This study provides certain basic data for the in-depth study of the comparison between dairy and beef cattle in the growth and development stages. |
| Key words: liver diary cattle beef Short Time-Series Expression Miner growth |
