| 摘要: |
| 为研究人工组合复合菌系降解木质纤维素的效果以及降解过程中微生物群落变化特征。首先对天然复合菌系PLC-8进行分离纯化，随后将筛选出的5株细菌和2株真菌进行组合，构建3 种简化的复合菌系，分别为F1、F2和F3。随后，设计秸秆降解试验，利用DGGE方法测定3种复合菌在分解纤维素过程中微生物群落的变化特征，并进行相关的理化分析。结果表明:人工组合复合菌系F3分解木质纤维素的能力最强，在分解木质纤维素30 d内秸秆平均减重率为39.80%，半纤维素下降52.64%，纤维素下降25.8%，木质素下降6.26%。在第15 天时纤维素内切酶、纤维素外切酶、滤纸酶、β-葡萄糖苷酶、木聚糖酶的活性已经达到整个分解过程的最高值，分别为0.09、0.08、0.09、0.07和0.11 U/mL。复合菌系F3能够自我调节pH，从而改变溶液的pH从微酸性至微碱性，F3的微生物群落呈现出真菌-细菌稳定共存的状态，并且能够长时间保持稳定。因此，人工组合复合菌系F3是稳定、高效的木质纤维素降解复合菌系。 |
| 关键词: 木质纤维素 复合菌系 人工组合 生物降解 |
| DOI：10.11841/j.issn.1007-4333.2022.11.14 |
| 投稿时间：2022-06-10 |
| 基金项目:科技部十三五科技支撑计划(2015BAD21B04)；吉林省科技厅项目(20200403028SF,20200402040 NC,YDZJ202201ZYTS578)；吉林省教育厅项目(JJKH20191130KJ,202201ZYTS578)；吉林省教育厅项目(JJKH20191130KJ) |
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| Characterization of lignocellulose decomposition by artificially combined composite strains |
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YANG Mengya1,WANG Yunlong1,AN Xiaoya1,ZHANG Liqiang1,CHEN Di1,PIAO Renzhe1,CUI Zongjun2,ZHAO Hongyan1*
|
| (1.College of Agriculture, Yanbian University, Yanji 133001, China;2. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| The aims of this study were to investigate the effect of the artificial combination of composite strains in degrading lignocellulose and the characteristics of microbial community changes during the degradation process. A natural composite strains PLC-8 was firstly isolated and purified, and then five bacteria strains and two fungi strains were combined to construct three simplified composite strains, which were F1, F2, and F3, respectively. Straw degradation experiment was designed to determine the characteristics of microbial community changes in the process of cellulose decomposition by using DGGE method and related physicochemical analysis. The results showed that: The artificial combination of complex strain F3 had the strongest ability to decompose lignocellulose, and the average weight loss of straw was 39. 80%, hemicellulose decreased by 52. 64%, cellulose decreased by 25. 8%, and lignin decreased by 6. 26% within 30 d of decomposing lignocellulose. The activities of cellulose endonuclease, cellulose exonuclease, filter paper activity, β-glucosidase and xylanase reached the highest values for the whole decomposition process on day 15, which were 0. 09 U/mL, 0. 08 U/mL, 0. 09 U/mL, 0. 07 U/mL, and 0. 11 U/mL, respectively. The complex strains F3 was able to self-regulate pH, thus it could change the pH of the solution from weak acidity to weak alkalinity. Therefore, the microbial community of F3 showed a stable coexistence of fungi-bacteria and was able to maintain stability for a long time. In conclusion, the artificial combination of complex strains F3 is a stable and efficient lignocellulose-degrading complex microbial system. |
| Key words: lignocellulose complex strain system artificial combination biodegradation |
