| 摘要: |
| 为初步揭示螺丝椒(Capsicum annuum L.)果实螺旋生长的机理，以螺丝椒与牛角椒自交系为试材，利用徒手切片、石蜡切片和转录组测序等技术，对试材果形、子房壁、果肉细胞分裂与膨大情况以及花期子房转录组差异进行了解析。结果表明:果实螺旋生长的起始可能发生在子房发育早期；螺丝椒的螺旋生长由果肉细胞倾斜排列所导致；两试材果肉在纵、横向细胞数与细胞大小方面存在差异，推测两试材果形差异是由细胞分裂与膨大综合调控的。转录组分析发现:螺丝椒果实可能通过改变KIN、NPH3和IQ67通路相关基因的表达使子房细胞分裂方向发生改变并最终导致果实呈现螺旋状生长，同时该试材果实还可能通过调控OFP8、IQ67通路相关基因以及细胞分裂素相关基因的表达来增加纵向与横向细胞数目并影响细胞形状，最终使果实伸长。综上，本研究可为揭示辣椒果形形成机理以及植物形态建成机理提供科学依据。 |
| 关键词: 辣椒 果形 螺旋 组织学 转录组 |
| DOI：10.11841/j.issn.1007-4333.2022.09.09 |
| 投稿时间：2022-02-07 |
| 基金项目:中国农业大学三亚研究院入库项目(SYND-2022-25)；前沿交叉学科发展与乡村振兴项目(4573-2421007)；中国农业大学2115人才资助工程(00109017) |
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| Histological and transcriptomic analysis of fruit rotation in pepper |
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CAO Xiaomeng,WANG Yixin,LI Zixiong,SHEN Huolin,SUN Liang
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| (College of Horticulture/Beijing Key Laboratory of Growth and Development Regulation of Protected Vegetables, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| The aim of this study was to illustrate the mechanism of the spiral growth characteristic in the helical-shaped pepper(Capsicum annuum L. ). Two inbred pepper lines, helical-shaped pepper and horn-shaped pepper, were taken as study objects. Freehand section method, paraffin section method and transcriptome sequencing were used. Mature fruit shape, cell division and expansion of ovary wall and pulp, and the transcriptome differences in ovary at anthesis were investigated. The results showed that: The onset of the spiral growth occurred in the early stages of ovary development. The spiral growth of pepper was attributed to the inclined arrangement of the cells in the ovary wall. In addition, significant differences were also observed in cell number and size in the longitudinal and transverse directions between the two lines. It is speculated that the difference of fruit shape between the two samples is comprehensively regulated by cell division and expansion. Transcriptomic analysis showed that, the expression of KIN, NPH3 and some of IQ67 pathway related genes was changed in the anthesis ovaries of the helical-shape fruit line, which may change the division direction of ovary cells and eventually lead to the spiral growth of fruit. Meanwhile, the helical-shaped fruits may also increase the number of longitudinal and transverse cells and affect the shape of cells by regulating the expression of OFP8 and the IQ67 pathway related genes and as well as the cytokinin-related genes, and finally elongated the helical-shaped fruits. In conclusion, the results of this study can provide scientific references for revealing the mechanism of the spiral growth of helical-shaped pepper and also for deepening our understanding about the mechanisms of plant organ morphogenesis. |
| Key words: pepper fruit shape rotation histology transcriptome |
