| 摘要: |
| 为筛选多抗优质樱桃番茄种质资源，利用PARMS(Penta-primer amplification refractory mutation system)技术对59个樱桃番茄种质资源，共696份番茄材料进行11个抗病基因的检测。结果表明:含有Ty-1抗病基因的资源约占35.3%，含有Ty-2抗病基因的资源约占17.8%，而含有I-2、I-3和Sw-5的抗病基因的植株仅占全部植株的5.9%、3.2%和5.2%，资源较为稀缺；对植株抗病基因的个数进行统计，选出25个代表性多抗(三抗及以上)樱桃番茄资源；对筛选出的多抗资源进行营养品质、风味品质和贮藏品质分析，综合抗病性、可溶性固形物含量、总酸含量、固酸比及外观品质，筛选出多抗优质资源6份:180-4、179-7、176-6、165-6、205-10和168-5；综合抗病性、番茄红素含量及外观品质，筛选出多抗、番茄红素含量高的资源6份:177-9、219-11、192-2、202-3、214-4和216-5。本研究筛选的资源可为多抗优质樱桃番茄新品种的选育提供资源基础。 |
| 关键词: 樱桃番茄 抗病基因检测 品质鉴定 筛选 |
| DOI：10.11841/j.issn.1007-4333.2022.08.11 |
| 投稿时间：2021-06-16 |
| 基金项目:河北省重点研发计划项目(21326309D)；河北省农林科学院财政项目(03-01-04)；现代农业产业技术体系河北省创新团队建设专项(HBCT2018030204)；河北省第三批“巨人计划”——蔬菜科研创新团队项目；石家庄市科学技术研究与发展计划项目(201490112A) |
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| Identification and screening of multiple resistance and high quality cherry tomato resources |
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WANG Shanshan1,YANG Chaosha2,YIN Weiping1,WANG Guohua1,ZHANG Liyong3,YIN Qingzhen1*
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| (1.Institute of Cash Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China;2.Chengde Academy of Agriculture and Forestry Sciences, Chengde 067055, China;3.Hebei Chemical and Pharmaceutical College, Shijiazhuang 050026, China) |
| Abstract: |
| In order to identify high-quality disease-resistant cherry tomato resources, the PARMS method was used in this research to analyze the distribution of eleven resistant(R)genes in 696 tomato materials of 59 cherry tomato germplasm resources. The results showed that: Most resources contain Ty-1 or Ty-2 gene, encompassing about 35. 3% and 17. 8% plants, respectively. On the contrary, the distribution of I-2, I-3 and Sw-5 genes are rare, which only present in 5. 9%, 3. 2% and 5. 2% of the total plants, respectively; A total of 25 resources with more than three R genes were selected as multiple resistant resources. Furthermore, the nutritional, flavor, and storage qualities of the selected multiple resistant resources were analyzed. According to disease resistance, their total soluble solid(TSS)content, total acid content, sugar-acid ratio, and appearance quality, six high-quality resources with multiple resistance were identified, including 180-4, 179-7, 176-6, 165-6, 205-10 and 168-5. Meanwhile, based on their disease resistance, lycopene content, and appearance quality, six resources with high lycopene content and multiple resistance were selected, which were 177-9, 219-11, 192-2, 202-3, 214-4 and 216-5. In conclusion, the identified resources in this research provide materials for the breeding of new cherry tomato varieties with multiple resistance and high quality. |
| Key words: cherry tomato resistant genes detection quality analysis screening |
