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| 芥蓝TCP家族全基因组鉴定及表达分析 |
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曾家晶1,2,赵怡娇1,2,曾东琳1,2,唐为玲1,2,李尊文1,Gefu WANG-PRUSKI2,赖钟雄1,郭容芳1,2*
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| (1.福建农林大学园艺植物生物工程研究所, 福州 350002;2.福建农林大学戴尔豪西大学联合试验室, 福州 350002) |
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| 摘要: |
| 为明确芥蓝TCP家族相关基因在叶片发育中的功能，基于甘蓝基因组数据，分析鉴定出芥蓝TCP基因家族有40个成员，参考拟南芥同源TCP基因注释及拟南芥数据库基因的相对表达量，初步选取BoTCP21和BoTCP25基因，采用qRT-PCR分析。结果表明:BoTCP21和BoTCP25均在叶片中大量表达，但二者在真叶不同部位的表达模式存在差异。为了进一明确TCP基因家族中参与叶片发育的基因，采用生物信息学方法并结合qRT-PCR对BoTCP家族进行了全面分析。结果显示:40个BoTCP都属于非分泌亲水性蛋白，分别定位在8条染色体上；系统进化树构建和亲缘关系分析将芥蓝中的BoTCP成员归为3类，其中PCF亚家族有19个成员，CYC亚家族8个成员，CIN亚家族13个成员。qRT-PCR结果表明:BoTCP21和BoTCP25在真叶和薹叶中具有较高的表达量，BoTCP14在开花结荚的植株根部表达量较高，而BoTCP16则在抽薹植株的根部表达量最高，说明BoTCP家族成员在组织部位表达存在时空特异性且广泛参与了植株的形态建成和器官发育。 |
| 关键词: 芥蓝 TCP基因家族 全基因组鉴定 生物信息学 表达分析 |
| DOI：10.11841/j.issn.1007-4333.2022.08.08 |
| 投稿时间：2021-10-21 |
| 基金项目:国家自然科学基金项目(31772310,31401859)；博士后启动经费(132300155)；博士后第10批特别资助(2017T100464) |
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| Genome identification and expression analysis of TCP family in Chinese kale |
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ZENG Jiajing1,2,ZHAO Yijiao1,2,ZENG Donglin1,2,TANG Weiling1,2,LI Zunwen1,Gefu WANG-PRUSKI2,LAI Zhongxiong1,GUO Rongfang1,2*
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| (1.Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2.Joint FAFU-Dalhousie Lab, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China) |
| Abstract: |
| In order to clarify the function of TCP family related genes in Chinese kale in leaf development, based on cabbage genome data, this study identified 40 members of TCP gene family in Chinese kale. Referring to the annotation of homologous TCP genes in Arabidopsis and the relative expression of genes in Arabidopsis database, BoTCP21 and BoTCP25 genes were preliminarily selected and analyzed by qRT-PCR experiment. The results showed that BoTCP21 and BoTCP25 were widely expressed in leaves, but their expression patterns were different in different parts of true leaves. In order to find out the genes involved in leaf development in TCP gene family, the BoTCP family was comprehensively analyzed by bioinformatics and qRT-PCR experiment. The results showed that 40 BoTCP were non-secreted hydrophilic proteins, which were located on 8 chromosomes respectively. The members of BoTCP in Chinese kale are classified into three categories by phylogenetic tree construction and phylogenetic analysis, among which there are nineteen members in PCF subfamily, eight members in CYC subfamily and thirteen members in CIN subfamily. The results of qRT-PCR showed that BoTCP21 and BoTCP25 had higher expression levels in true leaves and bolting leaves, BoTCP14 had higher expression levels in the roots of flowering and podding plants, while BoTCP16 had the highest expression levels in the roots of bolting plants, which indicated that the expression of BoTCP family members in the tissue parts was spatio-temporal specific and widely participated in the morphogenesis and organ development of plants. |
| Key words: Chinese kale TCP gene family whole genome identification bioinformatics expression analysis |
