| 摘要: |
| ADH基因在甜菜色素合成酪过程氨酸途径中发挥着重要作用，为探究苋菜酪氨酸途径的关键基因AtrADH2在苋菜中的功能，本研究以‘苏苋1号’‘苏苋2号’苋菜品种为试验材料，利用RT-PCR技术等研究方法克隆获得1个AtrADH2基因(GenBank登录号:MT982371)。结果表明:1)苋菜AtrADH2基因的ORF长为1 197 bp，编码398个氨基酸；2)AtrADH2蛋白属于NADB_Rossmann超级家族，含有1个NADB保守结构域；3)系统进化树分析表明，AtrADH2蛋白与甜菜BvADHα蛋白亲缘关系最近；4)‘苏苋1号’中甜菜色素含量受到光周期的影响；与其它组织部位相比，叶中的甜菜色素含量最高；与幼苗期相比，开花期叶和茎的甜菜色素含量均有所下降；5)qRT-PCR分析表明，AtrADH2基因表达趋势与AtrCYP76AD1、AtrDODA和AtrMYB1基因表达趋势相反。综上，AtrADH2基因可能对苋菜甜菜色素合成具有负调控作用，为进一步研究AtrADH2基因的功能提供了参考依据。 |
| 关键词: 苋菜 AtrADH2基因 甜菜色素 光周期 表达分析 |
| DOI：10.11841/j.issn.1007-4333.2022.04.10 |
| 投稿时间：2021-06-29 |
| 基金项目:福建农林大学优硕培育基金项目(1121YS01005)；福建省自然科学基金项目(2018 J01700)；福建农林大学科技创新专项基金(CXZX2017174, CXZX2017189) |
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| Cloning and expression analysis of AtrADH2 gene in Amaranthus tricolor L. |
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XIAO Fang1,WANG Le1,ZHENG Youfeng1,LAI Zhongxiong1,LIU Shengcai1*,MOU Jianmei2
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| (1.Institute of Horticultural Plant Bioengineering, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2.Suzhou City Academy of Agricultural Sciences, Suzhou 215000, China) |
| Abstract: |
| ADH gene plays an important role in the tyrosine pathway in the process of betalain synthesis. To explore the function of AtrADH2, a key gene of tyrosine pathway in amaranth, this study takes ‘Su Amaranth 1' and ‘Su Amaranth 2' as the test material. AtrADH2 gene(GenBank accession number: MT982371)was cloned by RT-PCR technology and other research methods. The results show that 1)AtrADH2 gene contains an ORF of 1 197 bp that encoding 398 amino acids; 2)AtrADH2 protein belongs to NADB_Rossmann superfamily, and containing a conserved domain of NADB; 3)Phylogenetic tree analysis shows that AtrADH2 protein is relatively close to the BvADHα protein; 4)Its betalain content is affected by the photoperiod in ‘Su Amaranth 1'. Compared with other tissues, the betalain content is the highest in leaf. Compared with the seedling period, the betalain content decreases in both leaf and stem at flowering period; 5)qRT-PCR analysis shows that AtrADH2 gene expression trend is opposite to AtrCYP76AD1, AtrDODA, AtrMYB1 gene expression betalain synthesis in amaranth, and provide a reference for further research on the function of AtrADH2. |
| Key words: Amaranthus tricolor L. AtrADH2 gene betalain photoperiod expression analysis |
