| 摘要: |
| 为明确乙烯对禾谷炭疽菌(Colletotrichum graminicola)致病性的影响并评价转BtACO基因拟南芥对禾谷炭疽菌的抗性。通过施加不同浓度外源乙烯利，对禾谷炭疽菌生长状况进行比较，并对喷施不同浓度乙烯利处理的野生拟南芥接种禾谷炭疽菌，分析禾谷炭疽菌的致病性，最后对转BtACO基因拟南芥接菌后的抗病性进行评价。结果表明:不同外源乙烯利浓度对禾谷炭疽菌黑色素及孢子生长的影响不同:10~100 mg/L乙烯利促进黑色素的产生，125~200 mg/L乙烯利抑制黑色素的产生；50~75 mg/L乙烯利对禾谷炭疽菌孢子萌发有抑制作用，高浓度乙烯利(125~200 mg/L)促进孢子的萌发。对野生型拟南芥接菌观察发现，高浓度乙烯利(125~200 mg/L)增强了禾谷炭疽菌的致病性；而转BtACO基因拟南芥较野生型植株发病程度轻，有更高的生物量积累。综上，低浓度乙烯利处理(10~75 mg/L)可降低禾谷炭疽菌的致病性，BtACO基因可以作为候选基因运用于作物对禾谷炭疽菌的抗性育种。 |
| 关键词: 禾谷炭疽菌 乙烯 致病性 BtACO基因 抗性 |
| DOI：10.11841/j.issn.1007-4333.2022.03.05 |
| 投稿时间：2021-09-05 |
| 基金项目:转基因生物新品种培育重大专项(2016ZX08003-001) |
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| Effects of exogenous ethephon on the biological characteristics of Colletotrichum gloeosporioides and disease resistance evaluation of BtACO plants |
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YANG Liyan1,GUO Xiaodi1,ZHANG Yurong1,BIAN Lu1,LIU Jinxiang2
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| (1.Life Science College, Shanxi Normal University, Taiyuan 030031, China;2.Citrus Research Institute, Southwest University, Chongqing 400712, China) |
| Abstract: |
| To explore the effects of ethylene on the pathogenicity of Colletotrichum graminicola and evaluate the resistance of BtACO Arabidopsis to C. graminicola, the growth profile of C. graminicola inoculated with different concentrations of exogenous ethephon was compared, and pathogenicity of C. graminicola was analyzed after being treated with ethephon. This study further investigated the resistance of BtACO Arabidopsis plants after being inoculated with C. graminicola. The results showed that different concentrations of exogenous ethephon had different effects on the production of melanin and spore. The 10-100 mg/L exogenous ethephon promoted the production of melanin while 125-00 mg/L ethephon inhibited melanin production. The 50-75 mg/L ethephon inhibited sporulation, while 125-200 mg/Lethephon promoted sporulation. The observation of the wild type Arabidopsis plants that inoculated with C. graminicola showed that 125-200 mg/L ethephon enhanced the pathogenicity of C. gloeosporioides. Compared with the wild type Arabidopsis, the BtACO plants were less infected and displayed higher biomass accumulation. To sum up, application of the lower concentration(10-75 mg/L)of exogenous ethephon could reduce pathogenicity of C. graminicola and BtACO could be used as a candidate gene to improve crops resistance to C. graminicola. |
| Key words: Colletotrichum graminicola ethylene pathogenicity BtACO resistance |
