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| 选择性多聚腺苷酸化及microRNAs对绵羊ACSL1基因表达的影响 |
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曹阳1,2,张立春1,于永生1,马惠海1,刘宇1,曹阳1*
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| (1.吉林省农业科学院 动物生物技术研究所, 吉林 公主岭 136100;2.浙江省农业科学院 畜牧兽医研究所, 杭州 310021) |
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| 摘要: |
| 为探究长链脂酰辅酶A合成酶(Long-chain acyl-CoA synthetases，ACSLs)在绵羊脂肪组织的表达规律，利用cDNA末端快速扩增技术(RACE)和双荧光素酶活性检测系统等方法进行研究。结果表明:1)绵羊ACSL1基因因选择性多聚腺苷酸化(Alternative polyadenylation，APA)存在2个不同长度的3′UTR；2)较短的3′UTR在绵羊前体脂肪细胞诱导分化前期更活跃，并且短3′UTR更有助于ACSL1蛋白表达；3)miR-202、miR-449a、miR-124a、miR-218均可与ACSL1 3′UTR结合，降低荧光素酶活性，其中miR-218通过与ACSL1 3′UTR的1 264~1 271 bp处结合可显著降低ACSL1表达。综上，ACSL1基因因选择性多聚腺苷酸化存在不同长度的3′UTR，且短3′UTR有助于基因表达；miR-218可显著下调ACSL1基因表达。本研究为绵羊ACSL1基因的进一步研究提供理论依据。 |
| 关键词: 选择性多聚腺苷酸化 ACSL1 microRNA 基因表达 |
| DOI：10.11841/j.issn.1007-4333.2022.01.12 |
| 投稿时间：2021-03-30 |
| 基金项目:吉林省自然科学基金项目(20190201161 JC)；财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS38) |
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| Effects of alternative polyadenylation and microRNAs on ACSL1 gene expression in sheep |
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CAO Yang1,2,ZHANG Lichun1,YU Yongsheng1,MA Huihai1,LIU Yu1,CAO Yang1*
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| (1.Institute of Animal Biotechnology, Jilin Academy of Agricultural Science, Gongzhuling 136100, China;2.Institute of Animal Husbandry and Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China) |
| Abstract: |
| In order to investigate the expression of long-chain acyl-CoA synthetase(ACSLs)in sheep adipose tissue, cDNA end rapid amplification technology(RACE)and dual luciferase activity detection system were used. The results showed that: 1)There were two different length 3′UTRs in sheep ACSL1 due to alternative polyadenylation(APA). 2)The shorter 3′UTR was more active in the early differentiation of sheep preadipocytes, and was more conducive to the expression of ACSL1 protein. 3)MiR-202, miR-449a, miR-124a and miR-218 can combine with ACSL1 3′UTR to reduce dual luciferase activity. MiR-218 can significantly reduce ACSL1 expression by binding to the longer 3′UTR of ACSL1 at 1 264-1 271 bp. In general, ACSL1 gene contains 3′UTRs of different lengths due to alternative polyadenylation. The short 3′UTR has a positive effect in the process of gene expression; miR-218 plays an important role in regulating ACSL1 gene expression. This study provides more theoretical basis for the study of sheep ACSL1 gene. |
| Key words: APA ACSL1 microRNA gene expression |
