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| 萱草属DUS测试中数量性状的测量方法研究 |
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褚云霞1,2,3,何玉祥4,邓姗1,2,陈海荣1,2,3*,任丽1,2,章毅颖1,2,赵洪1,2,李寿国2
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| (1.上海市农业科学院 农产品质量标准与检测技术研究所, 上海 201403;2.农业农村部植物新品种测试(上海)分中心, 上海 201415;3.上海市设施园艺技术重点实验室, 上海 201403;4.江西农业大学 生物科学与工程学院, 南昌 330045) |
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| 摘要: |
| 为了减小特异性、一致性和稳定性(Distinctness，uniformity and stability，DUS)测试中的数量性状观测误差，提高测量精度，本研究以种植于上海的30个萱草品种为试材，比对了定植时间、测量时期和观测部位对DUS测试数量性状的影响，分析了数量性状间的相关性，探讨了通过其它性状推测花直径的可行性。结果表明:定植时间主要影响叶丛、叶片和花葶3个部位的数量性状；移栽会影响其性状表达，第2个生长季叶丛高度和直径、叶片长度和宽度、花葶长度和粗度及花数量均明显大于第1个生长季观测值，大部分性状第3个生长季观测值均等于或小于第2个生长季的观测值；随着花序向上开放，花葶长度、花葶粗度和花数量基本无显著差异，其余5个性状不断变小；叶相关数量性状在营养生长期与盛花期测量无显著差异；所有数量性状与2~11个其他性状间存在显著相关，3个数量性状就能区分所收集的30个品种；通过外花被片长度和花型可以推测花直径。综上，萱草DUS测试需在休眠根状茎定植后的第2个生长季进行观测，前3朵正常开放的花均可为观测对象，叶相关性状可以改在盛花期测量，该结果可为萓草属数量性状的准确采集提供参考。 |
| 关键词: 萱草属 数量性状 测试时期 花着生位置 |
| DOI：10.11841/j.issn.1007-4333.2022.01.07 |
| 投稿时间：2021-03-03 |
| 基金项目:农业农村部2019年度品种资源保护项目(111821301354052254) |
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| Study on the measurement of quantitative characteristics in DUS testing of Hemerocallis |
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CHU Yunxia1,2,3,HE Yuxiang4,DENG Shan1,2,CHEN Hairong1,2,3*,REN Li1,2,ZHANG Yiying1,2,ZHAO Hong1,2,LI Shouguo2
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| (1.Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;2.Shanghai Sub-Center for New Plant Variety Tests, Ministry of Agriculture and Rural Affairs, Shanghai 201415, China;3.Shanghai Key Lab of Protected Horticultural Technology, Shanghai 201403, China;4.College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China) |
| Abstract: |
| In order to reduce the observation error of quantitative traits in the DUS testing and improve the measurement accuracy, this study investigated the effects of planting time, measurement time, and loci of flower on the quantitative characteristics of DUS testing. A total of 30 Hemerocallis varieties were taken as study materials. The correlation between the quantitative characteristics was analyzed. The feasibility of deducing flower diameter from other characters was discussed also. The results showed that: Planting time mainly affectes the quantitative traits of foliage, leaf and scape. The transplantation of daylily rhizome would affect the expression of these traits. The foliage height, foliage width, leaf length, leaf width, scape length, scape thickness and flower number measured in the second growth cycle are significantly higher than the observations in the first growth cycle. The observation values of most traits of all varieties in the third growth cycle are equal to or less than those in the second growth cycle. With flower opening upwards, there is no significant difference in scape length, scape thickness and flowers number, while the rest 5 traits keep decreasing. There are no significant differences between measurement data of leaf-related quantitative traits measured during the vegetative period and the full-blossom period. All quantitative characteristics are significantly related to other 2-11 traits. Combination of three quantitative characteristics could distinguish the 30 varieties tested. The diameter of flower can be predicted by the length of outer perianth and flower type. Therefore, in the DUS tests of Hemerocallis, it is necessary to observe in the second growing cycle after the dormant rhizomes are planted. The first three normally opening flowers are suitable for measuring and it is operable to measure leaf-related traits in blooming period. This result can provide references for the accurate collection of quantitative traits of Hemerocallis. |
| Key words: Hemerocallis quantitative characteristics period location of flower |
