| 摘要: |
| 为科学利用现有板栗种质资源并对其进行高效管理和保存。本研究利用简单重复序列(SSR)标记，采用非加权算数平均聚类法(UPGMA)对来自12个省(市或自治区)的279份板栗种质进行多次聚类抽样。聚类抽样时将2种遗传相似系数(SM系数和Dice系数)和2种取样方法(随机取样法和位点优先取样法)分别组合获得不同样本群，再比较不同样本群的有效等位基因数(Ne)、Nei’s多样性指数(H)和Shannon’s信息指数(I)，研究构建板栗初级核心种质的适宜方法，并采用t检验和主坐标分析评价初级核心种质的代表性，结合表型特征对其进行确认。结果表明:应用位点优先取样法取得的种质比随机取样法具有更高的Ne、H和I；应用SM相似性系数构建的核心样本，其遗传多样性指标要优于Dice相似性系数；根据主坐标结合形态学指标分析，利用位点优先取样法和SM相似性系数经过3次聚类构建了68份板栗初级核心种质，保留了原种质24.37%的样品，Ne、H和I分别为1.539、0.329和0.502，均高于原种质各遗传多样性指标，能够较全面的代表整个板栗资源的遗传多样性。综上，基于SSR标记利用SM系数聚类，并结合位点优先取样法是构建板栗初级核心种质较适宜的方法。 |
| 关键词: 板栗 初级核心种质 SSR 遗传多样性 |
| DOI：10.11841/j.issn.1007-4333.2021.12.09 |
| 投稿时间：2021-03-01 |
| 基金项目:河北省农林科学院农业科技创新专项(2019-3-4)；河北省农林科学院青年基金项目(2018020106) |
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| Construction of primary core collection of Castanea mollissima Bl. using SSR molecular markers |
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ZHANG Xinfang,ZHANG Shuhang,LI Ying,GUO Yan,WANG Guangpeng
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| (Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, China) |
| Abstract: |
| To utilize the current chestnut germplasm resources scientifically and manage and preserve them efficiently. Taking 279 Castanea mollissima Bl. germplasm resources from 12 provinces(cities and autonomous regions)as original materials, the multi-times random sampling based on SSR molecular markers was conducted by using UPGMA cluster method. The allele-preferred sampling method and random sampling strategy were compared according to SM or Dice similarity coefficient. The effective number of alleles, Nei's gene diversity index and Shannon's information index of two core sample groups were compared to determine the optimum building method. T-test and the principle coordinate analysis method were used to confirm on the primary core collection. The results showed that: The allele preferred sampling method was more representative with higher values of genetic diversity index compared with the random sampling strategy; The method of stepwise clustering according to SM similarity coefficient was better than Dice similarity coefficient; The principal coordinate analysis and morphological traits analysis showed that the core collection constructed by allele preferred sampling method and according to SM similarity coefficient could represent genetic diversity of initial collection more comprehensively. The 68 core collection included 24. 37% germplasm samples of initial collection. The effective number of alleles, Nei's gene diversity index and Shannon's information index were respectively 1. 539, 0. 329 and 0. 502, which were higher than those of initial collection. In conclusion, the core collection could represent the genetic diversity of the whole chestnut resource fully. The studies showed that UPGMA cluster method according to SM similarity coefficient based on SSR molecular markers combined with allele-preferred sampling strategy was an appropriate method for the construction of primary core collection of chestnut. |
| Key words: Castanea mollissima BL. primary core collection SSR genetic diversity |
