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| 一株犬副流感病毒CPIV-BJ01株全基因组测序与其遗传变异分析 |
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刘子宁1,2,魏津3,汤新明1,2,梁琳1,2,崔尚金1,2*
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| (1.中国农业科学院 北京畜牧兽医研究所, 北京 100193;2.农业农村部兽用药物与诊断技术北京科学观测实验站, 北京 100193;3.中国兽医药品监察所, 北京 100081) |
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| 摘要: |
| 为分析本实验室分离的犬副流感病毒CPIV-BJ01株全基因组序列与其遗传变异情况，对CPIV-BJ01株全长进行分段扩增并测序，拼接获得全基因组序列，与GenBank发布的27株副流感病毒5型分别进行全基因组和包膜糖蛋白(F、SH和HN)核苷酸及氨基酸序列的相似性比对，分析其遗传进化关系。结果显示，CPIV-BJ01毒株与犬源PIV5 1 168-1亲缘关系最近，核苷酸相似性为99.9%，氨基酸相似性为99.8%。CPIV-BJ01的F和HN基因序列变化较为保守，核苷酸和氨基酸变化均在4%以内，其中F蛋白的氨基酸变化形成一处新的O-糖基化位点；CPIV-BJ01株的SH基因序列与其他演化分支毒株差异显著，核苷酸相似性为84.4%~97.0%，氨基酸相似性为31.8%~95.6%。综上，CPIV-BJ01株基因组序列与前人报道的毒株相比，在F、HN基因和部分非编码区存在核苷酸或氨基酸序列的改变，同时F蛋白存在糖基化修饰的改变，均为该毒株所特有，该研究结果丰富了中国CPIV流行株的基因组信息。 |
| 关键词: 犬副流感病毒 基因组测序 序列分析 包膜糖蛋白基因 |
| DOI：10.11841/j.issn.1007-4333.2021.11.16 |
| 投稿时间：2021-01-11 |
| 基金项目:中央级公益性科研院所基本科研业务费(2021-YWF-ZYSQ-16)；国家重点研发计划(2017YFD0501603, 2016YFD0501003)；中国农业科学院创新工程计划(ASTIP-IAS15) |
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| Complete genome sequencing and genetic variation analysis of an isolated canine parainfluenza virus strain CPIV-BJ01 |
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LIU Zining1,2,WEI Jin3,TANG Xinming1,2,LIANG Lin1,2,CUI Shangjin1,2*
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| (1.Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2.Beijing Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, Beijing 100193, China;3.China Institute of Veterinary Drug and Control, Beijing 100081, China) |
| Abstract: |
| To analyze the complete genome sequence and genetic variation of canine parainfluenza virus strain CPIV-BJ01 isolated in laboratory, the full length of CPIV-BJ01 strain was amplified and sequenced. The nucleotide and amino acid of the whole genome and envelope glycoproteins(F, SH and HN)of 27 parainfluenza virus type 5 strains from GenBank database were compared. The genetic and evolutionary relationships were analyzed. The results showed that CPIV-BJ01 strain had the closest genetic relationship with canine PIV5 1 168-1, with nucleotide similarity of 99. 9% and amino acid similarity of 99. 8%. The F and HN gene sequences of CPIV-BJ01 were relatively conservative. Their changes of nucleotide and amino acid were within 4%, among which the amino acid of F protein formed a new O-glycosylation site. The SH gene sequence of CPIV-BJ01 was significantly different from that of other evolutionary branches with nucleotide similarity of 84. 4%-97. 0% and amino acid similarity of 31. 8%-95. 6%. In summary, compared with the previously reported strains, the genomic sequence of CPIV-BJ01 strain had nucleotide or amino acid sequence changes in F, HN gene and some non-coding genes, and there are glycosylation modifications in F protein, which were unique of the CPIV-BJ01 strain. These results enriched the genome information of Chinese CPIV epidemic strains. |
| Key words: canine parainfluenza virus genome sequencing sequence analysis envelope glycoprotein gene |
