| 摘要: |
| 为了评估北京地区梨园健康状况并明确梨树感染病毒和类病毒的主要种类，本研究利用RT-PCR对北京市重要梨果生产区的157份样品进行苹果茎沟病毒(apple stem grooving virus，ASGV)、苹果茎痘病毒(apple stem pitting virus，ASPV)、苹果褪绿叶斑病毒(apple chlorotic leaf spot virus，ACLSV)和苹果锈果类病毒(apple scar skin viroid，ASSVd)的检测。RT-PCR的结果表明，ASGV的检出率最高，高达91.1%，其次为ASPV和ASSVd，检出率分别为59.2%和54.8%，ACLSV的检出率相对较低，为35.7%。统计分析发现，84.7%的梨树样品表现出复合侵染，其中受到ASGV+ASPV+ASSVd复合侵染的样品数量最多，占所检样品数的25.5%；受到ASGV+ASPV或ASGV+ACLSV侵染的样品各占11.5%。同一果园中不同‘京白梨’植株所感染的病毒种类也不尽相同。本研究明确了北京地区梨园感染病毒和类病毒的主要种类，为梨病毒病防控提供了理论依据。 |
| 关键词: 梨 病毒 类病毒 RT-PCR 复合侵染 |
| DOI：10.11841/j.issn.1007-4333.2021.11.09 |
| 投稿时间：2021-01-06 |
| 基金项目:国家基础研发计划项目(2019YFD1001803)；国家现代农业(梨)产业技术体系(CARS-28) |
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| Detection of major viruses and viroid in pear plants in Beijing area |
|
ZENG Qi,HUANG Wenting,CHI Hai,WANG Shengnan,JIANG Feng,LI Tianzhong,CHENG Yuqin
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| (College of Horticulture, China Agricultural University, Beijing 100083, China) |
| Abstract: |
| To evaluate the phytosanitary status of pear orchards and characterize the prevalence of viruses and viroids in pear plants in Beijing area, 157 pear samples collected from main pear-growing districts were detected for the presence of apple stem grooving virus(ASGV), apple stem pitting virus(ASPV), apple chlorotic leaf spot virus(ACLSV)and apple scar skin viroid(ASSVd)by RT-PCR. The results showed that all samples were tested positive for virus(es), among which 91. 1% of the samples were tested positive for ASGV, 59. 2% for ASPV and 54. 8% for ASSVd. In contrast, the infection rate of ACLSV(35. 7%)was relatively low. Up to 84. 7% of the samples showed mixed infections, among which the mix-infection of ASGV+ASPV+ASSVd was the most common. It accounted for 25. 5% of the samples, followed by the mix-infection of ASGV+ASPV 11. 5% or ASGV+ACLSV 11. 5%. Virus(including viroid)species detected in different pear plants of same cultivar ‘jingbaili' in the same orchard were not exactly the same. In conclusion, the prevalence of viruses and viroid in pear plants in Beijing area was determined in this study, which could provide a reference for controlling of pear viral diseases. |
| Key words: Pyrus spp. virus viroid RT-PCR mixed-infection |
