| 摘要: |
| 为进一步挖掘甘蔗栽培种及其亲缘野生种中内生菌的功能与价值，筛选出具有促进植物生长功能活性的微生物菌株，用于促进甘蔗产业的增收增产。以前期分离纯化获得的589 株甘蔗内生细菌为基础，筛选并鉴定具有固氮、溶磷、解钾等促生长特性的功能菌株。在此基础上，对菌株产生的有机酸、吲哚乙酸(IAA)和嗜铁素也进行了定量测定与种类划分。结果表明:共有12 株功能菌株被筛选得到，分别编号为B7、B9、C9、D5、E12、F5、G3、H5、J8、L9、M3、O9，其中多株菌株同时具有固氮溶磷解钾等生物学活性；12株菌株分别属于Pantoea、Pseudomonas、Enterobacter、Serratia、Acinetobacter和Bacillus 6个属。进一步功能研究结果表明:菌株G3与C9对于无机磷和有机磷的溶解能力较强，溶磷量分别为1 386.97±331.96与2 523±68.17 mg/L；菌株M3解钾能力较强，解钾量为8.59±0.08 mg/L；各菌株所产生的有机酸主要为酒石酸，其次为苹果酸、乳酸、乙酸、柠檬酸；12株功能菌株可产生IAA及嗜铁素，但产生嗜铁素的种类随菌株而异；各菌株与甘蔗种苗共培养过程中均能促进甘蔗种苗芽长与根长的生长，其中表现较优的有E12、C9、G3菌株处理组。该研究可为后续微生物菌肥开发奠定材料基础，也为相关研究提供参考。 |
| 关键词: 甘蔗 内生细菌 促生长 植物激素 有机酸 |
| DOI：10.11841/j.issn.1007-4333.2021.11.07 |
| 投稿时间：2021-04-01 |
| 基金项目:云南省现代农业甘蔗产业技术体系建设专项(2020-2021)；云南省研究生导师团队建设项目(2019YJSDSTD01) |
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| Screening, identification and biological function study of endophytic bacteria isolated fromsugarcane and its related genera |
|
DI Yining1,XIE Linyan1,GU Shujie1,MA Hao1,LIU Lufeng2,HE Lilian1,2,LI Fusheng1,2*
|
| (1.College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China;2.Sugarcane Research Institute, Yunnan Agricultural University, Kunming 650201, China) |
| Abstract: |
| To further investigate the function and value of endophytes in sugarcane cultivars and wild resources of Saccharum related species, and screened the microbial strains with the functional activity of promoting plant growth, in the hope of increasing sugarcane income and production. In this study, a total of 589 endophytic bacteria strains separated and purified previously were taken as research material. We screened and identified some functional strain which has ability of nitrogen fixation, uptake of insoluble phosphorus, potassium and other growth-promoting characteristics. On this basis, the organic acids, indole acetic acid(IAA)and siderophore produced by the strains were also quantitatively determined and classified. The results showed that the twelve endophytic bacteria strains were selected out, and these strains were numbered temporally as B7, B9, C9, D5, E12, F5, G3, H5, J8, L9, M3 and O9, respectively. Among them, most strains have biological activities such as nitrogen fixation, phosphorus dissolution and potassium solubilisation. Twelve strain were identified as belonging to six generas including Pantoea, Pseudomonas, Enterobacter, Serratia, Acinetobacter and Bacillus. Notably, the strain G3 and the C9 had the strongest ability to dissolve inorganic phosphorus and organic phosphorus, following tested, the concentration of soluble phosphorus in the medium were(1 386. 97±331. 96)mg/L and(2 523±68. 17)mg/L, respectively. The strain M3 had strong potassium-resolving ability and the concentration of soluble potassium in the culture solution was 8. 59±0. 08 mg/L. The organic acids produced by the strain mainly tartaric acid, and the rest were malic acid, acetic acid, lactic acid and citric acid. Twelve functional strains generally have the ability to produce IAA and siderophore, while the types of siderophore produced vary from strain to strain. Some strains can promote the growth of sugarcane seedling bud and root length when co-cultivation with sugarcane seedlings, among which E12, C9 and G3 treatment perform better. This research laid a material foundation for the subsequent development of microbial fertilizer in the future, and also provide reference for related research. |
| Key words: sugarcane endophytic bacteria growth-promoting phytohormone organic acid |
