| 摘要: |
| 为研究大豆滞绿突变对叶片衰老过程中蔗糖代谢相关基因表达产生的影响，以晋大滞绿1号及其亲本晋大74号为试验材料，测定了大豆开花后至成熟期间叶片可溶性糖含量的变化趋势，并对比了不同基因型大豆品种叶片衰老过程中蔗糖代谢相关基因的表达差异。结果表明:1)花后至成熟期间，2个供试大豆品种可溶性总糖含量均呈波动升高然后降低的变化趋势。花后14至42天，晋大滞绿1号可溶性总糖含量高于晋大74号；2)花后29至42天，晋大滞绿1号蔗糖磷酸合成酶基因SPS4，转化酶基因CInv、CWInv2以及蔗糖合酶基因SS1、SS2-2表达水平显著高于非滞绿品种晋大74号；己糖激酶和果糖激酶基因Hxk和Frk表达量也高于非滞绿品种晋大74号；3)除 SUT4-1外，其余6个蔗糖转运体基因SUTs的表达模式具有明显的品种特异性。花后29至42天，晋大滞绿1号叶片SUTs表达水平较高。综上，滞绿突变对于大豆蔗糖代谢相关基因的表达产生了明显的影响，特别是在鼓粒初期和中期等大豆籽粒形成的关键时期，滞绿品种晋大滞绿1号蔗糖代谢相关基因的表达更加活跃。 |
| 关键词: 大豆 滞绿 蔗糖代谢 基因表达 |
| DOI：10.11841/j.issn.1007-4333.2021.11.06 |
| 投稿时间：2021-03-14 |
| 基金项目:国家重点研发计划项目(2017YFE0111000)；欧盟H2020研究创新项目(727312)；山西农业大学青年创新项目(20142-03) |
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| Effects of stay-green mutation on the expression of sucrose metabolism-related genes in soybean leaves |
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WANG Peng1,HOU Siyu1,2,WEN Hongwei1,LI Guiquan1*
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| (1.College of Agriculture, Shanxi Agricultural University, Taigu 030801, China;2.Institute of Agricultural Biotechnology, Shanxi Agricultural University, Taigu 030801, China) |
| Abstract: |
| To study the effect of a stay-green mutation on the expression of sucrose metabolism-related genes during leaf senescence, taking Zhilv No. 1(Z1)and one of its parents Jinda No. 74(JD74)as experimental materials, the change trend of soluble sugar content in soybean leaves from flowering to maturity was determined and the expression differences of sucrose metabolism-related genes were compared. The results indicated that: 1)The soluble sugar content of two soybean varieties fluctuated, increased and then decreased during the period from anthesis to maturity. From 14 to 42 days after flowering, the content of soluble sugar was much higher in Z1 than that in JD74. 2)From 29 to 42 days after anthesis, the expression levels of sucrose phosphate synthase gene SPS4, invertase iso-genes CInv, CWInv2 and sucrose synthase iso-genes SS1 and SS2-2 in Z1 were significantly higher than those in JD74. The expression levels of hexokinase and fructokinase genes Hxk and Frk were also much higher than that in JD74. 3)Except for SUT4-1, sucrose transporter iso-genes SUTs showed obvious variety-specific expression patterns. From 29 to 42 days after anthesis, the expression levels of SUTs in Z1 were higher. In summary, the stay-green mutation in soybean had a significant impact on the expression of sucrose metabolism-related genes, especially in the key periods of soybean grain formation such as the early and middle stage of pod filling, the expression of source metabolism related genes in Z1 was more transcriptionally active. |
| Key words: soybean stay green sucrose metabolism gene expression |
