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| LED脉冲光对生菜叶绿素荧光参数的影响 |
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梅延豪1,刘齐1,李琦1,虞慧彬1,白雅晖1,徐晓东1,武永军2,杨振超1*
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| (1.西北农林科技大学 园艺学院, 陕西 杨凌 712100;2.西北农林科技大学 生命学院, 陕西 杨凌 712100) |
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| 摘要: |
| 为探究LED脉冲光对作物叶绿素荧光特性的影响，本研究以玻璃生菜为研究对象，设计了14个频率(1、2、4、8、16、32、64、128、256、512、1 024、2 048、4 096和8 192 Hz)和5个占空比(20%、40%、60%、80%和100%)组合的脉冲光，利用双通道PAM-100荧光仪测定了不同脉冲光处理下生菜叶片的叶绿素荧光参数。结果表明:低频率(<256 Hz)低占空比(≤60%)处理的光系统II(PSII)最大光量子效率(Fv/Fm)、有效光量子效率(Fv′/Fm′)、实际光化学量子产额(ФPSII)和光化学淬灭(qP)显著低于连续光，非光化学淬灭(NPQ)和PSII调节性能量耗散的量子产额(ФNPQ)显著高于连续光处理；2 Hz以下低占空比处理的非调节性能量耗散的量子产额(ФNO)显著高于连续光；256 Hz及以上和256 Hz以下的部分高占空比处理的荧光参数与连续光的荧光参数无显著差异。综上，低频率低占空比的脉冲光会降低生菜叶片的光合活性，2 Hz的低占空比会导致PSII发生损伤，高频率以及低频高占空比的脉冲光处理不会对生菜叶片的PSII活性、光合电子传递及能量分配造成影响。本研究可为LED节能补光灯的研发提供理论支撑。 |
| 关键词: 生菜 脉冲光 叶绿素荧光 频率 占空比 |
| DOI：10.11841/j.issn.1007-4333.2021.09.11 |
| 投稿时间：2020-10-29 |
| 基金项目:国家重点研发计划(2018YFD0201205-2)；陕西省农业厅农业科技创新集成推广项目(NYKJ-2018-YL22)；陕西省技术创新引导专项(2021-YD-QFY-0094) |
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| Effect of LED pulsed light on chlorophyll fluorescence parameters of lettuce |
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MEI Yanhao1,LIU Qi1,LI Qi1,YU Huibin1,BAI Yahui1,XU Xiaodong1,WU Yongjun2,YANG Zhenchao1*
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| (1.College of Horticulture, Northwest A & F University, Yangling 712100, China;2.College of Life science, Northwest A & F University, Yangling 712100, China) |
| Abstract: |
| In order to explore the effect of LED pulsed light on the chlorophyll fluorescence characteristics of crops, glass lettuce was taken as the research object. A total of 14 different frequencies(1, 2, 4, 8, 16, 32, 64, 128, 256, 512, 1 024, 2 048, 4 096 and 8 192 Hz)were designed and 5 duty ratios(20%, 40%, 60%, 80% and 100%)combined pulsed light. The chlorophyll fluorescence parameters of lettuce leaves under different pulsed light treatment with dual-channel PAM-100 fluorometer were measured. The results showed that: Photosystem II(PSII)biggest quantum efficiency Fv/Fm under the low frequency(<256 Hz)and low duty ratio(≤60%)processed, actual photochemical quantum yield ФPSII and photochemical quenching qP were all significantly lower than under continuous light. The non photochemical quenching NPQ and PSII regulatory quantum yield of energy dissipation ФNPQ wwere significantly higher than that of continuous light treatment; The quantum yield of non-regulatory energy dissipation ФNO treated with low duty ratio below 2 Hz significantly was higher than that of continuous light; There was no significant difference in fluorescence parameters between treatment of continuous light and treatment of 256 Hz or below in the high duty ratio and 256 Hz or above. In summary, the pulsed light with low frequency and low duty ratio could reduce the photosynthetic activity of lettuce leaves, and low duty ratio of 2 Hz could lead to PSII damage. The PSII activity, photosynthetic electron transfer and energy distribution of lettuce leaves were not affected by the pulsed light treatment with high frequency and low frequency high duty ratio compared with continuous light. This research can provide theoretical support for the research and development of LED energy saving supplementary light lamp. |
| Key words: lettuce pulsed light chlorophyll fluorescence frequency duty ratio |
