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| 苜蓿皂苷对X射线照射小鼠造血系统的影响 |
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张明1,2,李志威3,刘建宇1,张靖雪3,郭志鹏1,曲根1,严学兵3*,郭玉霞1*
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| (1.河南农业大学 牧医工程学院, 郑州 450002;2.濮阳职业技术学院, 河南 濮阳 457000;3.扬州大学 动物科学与技术学院, 江苏 扬州 225009) |
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| 摘要: |
| 为研究苜蓿皂苷(Alfalfa saponin)对X射线照射小鼠造血系统的影响，选取雄性SPF级昆明系小鼠90只，随机分为对照组和试验组，研究25、50和100 mg/ml的苜蓿皂苷对X射线照射强度为4.0 Gy小鼠造血系统的影响和不同剂量X射线对添加25 mg/ml的小鼠造血系统的影响。结果表明:1)预防性给予25 mg/mL苜蓿皂苷，可显著减轻4.0 Gy的X射线对小鼠外周血白细胞(White blood cells，WBC)、红细胞(Red blood cells，RBC)和血小板(Platelets，PLT)数量的降低程度(P<0.05)，增加照射小鼠的骨髓有核细胞(Bone marrow nucleated cells，BMC)数(P<0.05)，显著降低微核率(P<0.05)。2)预防性给予25 mg/mL的苜蓿皂苷，相同照射剂量的试验组小鼠白细胞、红细胞、血小板和骨髓有核细胞数显著高于对照组(P<0.05)，微核率显著低于对照组(P<0.05)。3)随照射强度增加，25 mg/ml的苜蓿皂苷保护小鼠受辐射损伤的能力下降。综上，苜蓿皂苷可缓解X射线对小鼠造血系统和染色体的损伤，苜蓿皂苷浓度与射线强度存在量效关系，需要根据不同的照射强度添加不同浓度的苜蓿皂苷。 |
| 关键词: 苜蓿皂苷 X射线 小鼠 造血系统 |
| DOI：10.11841/j.issn.1007-4333.2021.07.08 |
| 投稿时间：2020-08-19 |
| 基金项目:国家“十三五”重点研发计划项目(2017YFD0502104) |
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| Effect of alfalfa saponins on hematopoietic system of mice irradiated by X-ray |
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ZHANG Ming1,2,LI Zhiwei3,LIU Jianyu1,ZHANG Jingxue3,GUO Zhipeng1,Qu Gen1,YAN Xuebing3*,GUO Yuxia1*
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| (1.College of Animal and Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China;2.Puyang Vocational and Technical College, Puyang 457000, China;3.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China) |
| Abstract: |
| In order to study the effect of alfalfa saponin on the hematopoietic system of X-ray irradiated mice, 90 male SPF Kunming rice were selected and randomly divided into control group and experimental group. The effects of 25, 50 and 100 mg/mL alfalfa saponin on the hematopoietic system of mice with X-ray irradiation intensity of 4. 0 Gy and the effects of different doses of X-ray on the hematopoietic system of mice with X-ray irradiation intensity of 25 mg/mL of were studied. The results showed that: 1)Preventive usuage of 25 mg/mL alfalfa saponin could significantly reduce the decrease of white blood cells(WBC), red blood cells(RBC)and platelets(PLT)in peripheral blood of mice exposed to 4. 0 Gy X-ray(P<0. 05), and increased the bone marrow nucleated cells(BMN)in irradiated mice. The number of cells(BMC)decreased significantly(P<0. 05). 2)After prophylactic administration of 25 mg/mL alfalfa saponin, the number of white blood cells, red blood cells, platelets and bone marrow nucleated cells in experimental group with the same irradiation dose was significantly higher than that in control group(P<0. 05), and the micronucleus rate was significantly lower than that in control group(P<0. 05). 3)With the increase of radiation intensity, the protection ability of 25 mg/ mL alfalfa saponin against radiation injury in mice decreased. To sum up, alfalfa saponin could alleviate the damage of hematopoietic system and chromosome of mice caused by X-ray. There was a dose-effect correlation between the concentration of alfalfa saponin and the radiation intensity of X-ray. Different concentration of alfalfa saponin should be applied according to different X-ray radiation intensity. |
| Key words: alfalfa saponin X-ray mice hematopoietic system |
