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| NtCPS2基因对顺-冷杉醇与赤霉素合成代谢途径的影响 |
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贺凌霄1,何雷2,李洪臣3,李芳芳2,颜培强4,姚远4,薛刚1,孙聚涛1,杨铁钊1,徐世晓1*
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| (1.河南农业大学 烟草学院, 郑州 450002;2.中国烟草总公司 河南省公司, 郑州450046;3.河南省烟草公司 三门峡市公司, 河南 三门峡 472000;4.黑龙江省烟草公司 哈尔滨烟叶公司, 哈尔滨 150001) |
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| 摘要: |
| 为探讨顺-冷杉醇合成基因NtCPS2对萜类代谢途径的影响，利用基因编辑技术CRISPR/Cas 9编辑烟草顺-冷杉醇合成基因NtCPS2，并筛选出纯合突变植株，命名为‘8306’编辑系；比较‘8306’编辑系(T2-26、T2-45、T2-48)与野生型(WT)在顺-冷杉醇、赤霉素含量及合成关键基因的生物信息学方面的差异。结果表明:‘8306’编辑系(T2-26、T2-45、T2-48)顺-冷杉醇含量平均减少86.20%，赤霉素含量平均增加27.05%；顺-冷杉醇合成途径中的关键基因表达量显著减小，赤霉素合成途径中正调控关键基因GA20ox表达量显著增加，负调控关键基因GA2ox表达量显著减小，并且GGPP(牻牛儿基牻牛儿基焦磷酸)合成的关键基因DXR表达量增加。NtCPS2基因不仅影响赖百当类二萜代谢途径，有效降低顺-冷杉醇的含量，并且影响赤霉素萜类代谢，增加赤霉素的含量。 |
| 关键词: 烟草 ‘8306’编辑系 NtCPS2 冷杉醇 赤霉素 |
| DOI：10.11841/j.issn.1007-4333.2021.06.04 |
| 投稿时间：2020-06-12 |
| 基金项目:中国烟草总公司河南省公司重大专项(2018410000270035)；中国烟草总公司科技重点项目(110201002008)；中国烟草总公司科技重点项目(110201402004)；黑龙江省烟草行业科技开发项目(201723000100251) |
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| Effects of NtCPS2 gene on the anabolic pathways of cis-abienol and gibberellin |
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HE Lingxiao1,HE Lei2,LI Hongchen3,LI Fangfang2,YAN Peiqiang4,YAO Yuan4,XUE Gang1,SUN Jutao1,YANG Tiezhao1,XU Shixiao1*
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| (1.College of Tobacco, Henan Agricultural University, Zhengzhou 450002, China;2.China Tobacco Corporation Henan Company, Zhengzhou 450046, China;3.Sanmenxia Tobacco Company of Henan, Sanmenxia 472000, China;4.Harbin Tobacco Company of Heilongjiang, Harbin 150001, China) |
| Abstract: |
| To investigate the effect of cis-abienol synthesis gene NtCPS2 on terpenoid metabolic pathways, CRISPR/Cas 9 gene editing technology was used to edit the tobacco cis-abienol synthesis gene NtCPS2. The homozygous mutant plants were screened, and named as ‘8306' gene-editing series. The content and the key synthesis genes of cis-abienol and gibberellin were compared between the ‘8306' gene-editing series(T2-26, T2-45, T2-48)and the wild type(WT). Compared with WT, the cis-abienol content in the ‘8306' gene-editing series was decreased by 86. 20%, the gibberellin content was increased by 27. 05% on average. The key gene′s expression in the synthesis pathway of cis-abienol was significantly reduced. In the gibberellin synthesis pathway, the expression level of GA20ox, the key gene of positive regulation, was significantly increased. At the same time, the expression level of GA2ox, the key gene of negative regulation, was significantly decreased. Moreover, the expression level of DXR, the key gene of GGPP synthesis, was increased. In conclusion, the NtCPS2 gene not only affected the labdane diterpenes metabolic pathway and effectively reduced cis-abienol content, but also affected the gibberellin metabolism and increased gibberellin content. |
| Key words: tobacco the ‘8306' edited strains NtCPS2 cis-abienol gibberellin |
