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| TaSPX3基因VIGS沉默表达降低小麦对叶锈病(Puccinia recondite f. sp. tritici)的抗性 |
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张蕊1,李博1,李旭1,尚文静1,韩迎春2,程琨1,刘娜1*,郑文明1,2*
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| (1.河南农业大学 生命科学学院, 郑州 450002;2.河南农业大学 小麦玉米作物学国家重点实验室/河南粮食作物协同创新中心, 郑州 450002) |
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| 摘要: |
| 为挖掘TaSPX3基因在小麦抗叶锈病中的作用，本研究以前期对叶锈菌感染的小麦转录组数据分析发现的TaSPX3基因受到叶锈菌侵染诱导表达为依据，利用BSMV-VIGS系统沉默中国春和郑麦9023中的TaSPX3基因，利用qRT-PCR技术进一步分析小麦抗病相关基因的转录水平。结果发现TaSPX3基因沉默后，小麦叶锈菌感染加重，降低了小麦对叶锈病的抗性；同时，对侵染过程抗病相关基因转录检测发现，抗病相关基因PR2和CAT的表达水平显著下调。本研究表明TaSPX3基因可能通过调控PR2和CAT基因的表达参与小麦对叶锈病的抗性。 |
| 关键词: 小麦 TaSPX3 VIGS 叶锈病 抗病性 |
| DOI：10.11841/j.issn.1007-4333.2021.01.03 |
| 投稿时间：2020-05-18 |
| 基金项目:河南省自然科学基金项目(182300410023,202300410202)；河南省科技攻关项目(182102110278)；河南省教育厅重点项目(17B210003) |
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| Silencing the expression of TaSPX3 by VIGS decreased the resistance of wheat to leaf rust(Puccinia recondite f. sp. tritici) |
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ZHANG Rui1,LI Bo1,LI Xu1,SHANG Wenjing1,HAN Yingchun2,CHENG Kun1,LIU Na1*,ZHENG Wenming1,2*
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| (1.College of Life Science, Henan Agricultural University, Zhengzhou 450002, China;2.Collaborative Innovation Center of Henan Grain Crops/State Key Laboratory of Wheat and Maize Crop, Henan Agricultural University, Zhengzhou 450002, China) |
| Abstract: |
| In order to explore the function of TaSPX3 in wheat resistance to leaf rust based on the discover that TaSPX3 expression was induced by leaf rust infection, BSMV-VIGS system was used to silence TaSPX3 in Chinese Spring and Zhengmai 9023. qRT-PCR was adopted to analysis the expression of disease resistant genes. The results showed that: The disease severity of leaf rust on BSMV∶TaSPX3 infected wheat leaves was aggravated, which indicated that the resistance to leaf rust was reduced after silencing TaSPX3 gene; The expression levels of diseases-resistance genes PR2 and CAT were significantly down-regulated in the leaves. In conclusion, TaSPX3 might be involved in wheat resistance to leaf rust fungus by regulating the expression of PR2 and CAT genes. |
| Key words: wheat TaSPX3 VIGS leaf rust resistance |
