| 摘要: |
| 为了解规模化蛋鸡场舍内乳头式饮水系统细菌微生物污染情况，采用实时荧光定量PCR 法(Quantitative Real-time PCR)检测8层层叠式笼养蛋鸡舍内第1、3、5、7层水线水和V型槽水中细菌基因组16S rDNA总拷贝数，并与鸡产蛋性能和蛋品质性状进行相关性分析。结果表明:1)不同时间各笼层之间的水线水和V型槽水细菌总拷贝数均无显著差异(P>0.01)，所测每层的V型槽水细菌总拷贝数在各阶段均显著高于水线水(P<0.01)，高达46.77~1 905.46倍；2)第7笼层V型槽水细菌数最低，蛋鸡的产蛋率、蛋白高度和哈氏单位最高；3)水线水和V型槽水细菌总拷贝数在不同笼层均呈正相关(相关系数r=0.078~0.183)，V型槽水细菌总拷贝数与产蛋率和鸡蛋质量在第3、5、7层呈弱负相关(r=-0.305~-0.127，-0.164~-0.060)，与蛋白高度和哈氏单位在各笼层均呈弱负相关(r=-0.192~-0.037，-0.138~-0.047)。层叠式蛋鸡舍内V型槽水中细菌微生物含量远高于水线水中，污染程度相对较高，对蛋鸡健康、产蛋性能和蛋品质有较大的潜在负面影响。 |
| 关键词: 层叠式蛋鸡舍 乳头式饮水 V型水槽 细菌含量 产蛋性能 |
| DOI：10.11841/j.issn.1007-4333.2020.07.13 |
| 投稿时间：2019-12-11 |
| 基金项目:国家蛋鸡产业技术体系(CARS-40-K21)；安徽省科技重大专项(18030701172, 17030701064)；安徽省自然科学基金项目(1908085QC115) |
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| Detection of bacterial content in the nipple watering system of layer house with cascading cages in summer |
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WAN Yi1,SHENG Huanyu2,MA Ruiyu1,DU Qiang3,YANG Rongbin3,ZHAN Kai1*
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| (1.Anhui Key Laboratory of Livestock and Poultry Product Safety Engineering/Institute of Animal Husbandry andVeterinary Medicine, Anhui Academy of Agriculture Science, Hefei 230001, China;2.College of Animal Science and Technology, Anhui Agricultural University, Hefei 230001, China;3.Anhui Sundaily Ecological Food Co. Ltd., Tongling 244100, China) |
| Abstract: |
| In order to investigate the microbial pollution in the nipple watering system in the layer houses, the bacterial contents in the pipe water and the V-trough on the 1st, 3rd, 5th, 7th tier of the layer house with eight overlap tiers were continuously monitored, respectively. The bacterial contamination and its association with laying performance and egg quality traits were compared. The number of bacterial genome 16S rDNA copies was detected by real-time PCR. The results showed that: There was no significant difference in the number 16S rDNA copies in both pipe water and V-trough among tiers(P>0. 01), while the number in the V-trough was 46. 77-1 905. 46 times higher than that in the pipe water(P<0. 01)of each tier throughout the experiment. The V-trough on the 7th tier had the lowest 16S rDNA copy number of bacteria, but had the highest laying rate, albumen height and Haugh unit of the layers. The 16S rDNA copy number of bacteria in the pipe water was positively correlated with that in the V-trough on different tiers(correlation coefficient, r=0. 078-0. 183). The 16S rDNA copy number in V-trough was weakly negatively correlated with laying rate and egg weight on the 3rd, 5th and 7th tier(r=-0. 305 - 0. 127, -0. 164 - 0. 060), and weakly negatively correlated with albumen height and Haugh unit(r =-0. 192 - 0. 037, -0. 138 - 0. 047)on each tier. In conclusion, the bacteria content in the V-trough was much higher than that in the pipe water indicating a higher level of pollution, which might have potential negative impacts on the health, laying performance and egg quality of laying hens. |
| Key words: layer house nipple watering system V-trough bacteria content laying performance |
