| 摘要: |
| 为挖掘新的抗南方锈病基因资源，本研究以甜玉米组合 M5×M114 的216 个 F2单株为遗传作图群体，应用 BSA 方法从500 对 SSR引物中筛选出2 对在 F2代抗病和感病 DNA 池间具有多态性的引物，分别位于4和9号染色体上；在4和9号染色体上重新设计100对 SSR 引物，构建了包含33 个标记位点总长为241.2 cM 的连锁遗传图，各个标记间的平均距离为7.53 cM。结合 F2 单株对南方锈病的抗性表现，用复合区间作图法在4和9号染色体上共检测到7 个显著的南方锈病抗性QTLs，其中:4个QTLs位于4号染色体上，可解释12.1%、7.8%、18.2%和14.9%表型变异；3个位于9号染色体上，分别解释17.0%、13.3%与19.2%的表型变异。研究结果可为抗南方锈病的精细定位、主效基因克隆和抗南方锈病鲜食甜玉米品种选育提供理论依据。 |
| 关键词: 甜玉米 南方锈病 群体分离分析 QTL作图 |
| DOI：10.11841/j.issn.1007-4333.2020.04.09 |
| 投稿时间：2019-10-07 |
| 基金项目:广东省鲜食玉米遗传育种工程中心(粤科产学研［2016］176号)；广东省农业厅种业提升项目(粤财农［2014］492号)；广东省重点领域研发计划(2018B020202013)；广州国家现代农业产业科技创新中心创建项目(2018KCZX02) |
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| QTL mapping for Southern rust resistance in fresh sweet corn |
|
ZHANG Jinyu1,PU Haojie2,LIU Pengfei1,CHEN Qingchun1,ZHANG Zili1,JIANG Feng1*
|
| (1.College of Agronomy and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;2.Yunfu Yuncheng District Agricultural Rural and Water Bureau, Yunfu 527300, China) |
| Abstract: |
| To explore new genetic resources to Southern rust in this study, 216 F2 individuals of sweet corn combination M5×M114 were used as genetic mapping population to scan resistant QTL of maize Southern rust with the method of Bulked Segregant Analysis. The results showed that: Over 500 SSR markers screened, two markers located on chromosomes 4 and 9 showed polymorphic between resistant and susceptible DNA pools. A linkage genetic map containing 33 marker loci with a total length of 241. 2 cM was constructed by redesigning 100 pairs of SSR primers on chromosomes 4 and 9, and the average distance between individual markers was 7. 53 cM. Combined with the resistance index of 216 F2 families, a total of 7 significant Southern rust resistance QTLs were detected on chromosomes 4 and 9 by composite interval mapping. Four QTLs were located on chromosome 4 and respectively explained 12. 1%, 7. 8%, 18. 2% and 14. 9% of phenotypic variation. And 3 significant QTLs were located on chromosome 9, which explained 17. 0%, 13. 3% and 19. 2% of the phenotypic variation, respectively. This study provided a theoretical basis for QTL fine mapping of Southern rust resistance, cloning of major genes and breeding Southern rust resistant varieties for maize. |
| Key words: sweet corn Southern rust bulked segregant analysis QTL mapping |
