| 摘要: |
| 利用筛选出的21对SSR引物对7个燕山板栗群体142份资源和1个太行山板栗群体9份资源进行遗传多样性分析，并构建不同群体的聚类树状图和主坐标分析图，旨在为燕山板栗种质资源的遗传背景明晰和创新利用提供依据。结果表明:21对引物共检测到71个等位变异位点，变异范围为2~6，平均每对SSR引物可检测到3.38个等位位点；多态性信息含量为0.614 5~0.972 3，平均0.866 8。8个板栗群体有效等位基因数Ne、Nei’s 多样性指数H、Shannon’s 信息指数I、总遗传多样性指数Ht、群体内遗传多样性指数Hs分别为1.457 8、0.309 9、0.468 0、0.306 2和0.291 2，说明燕山板栗群体的遗传多样性和变异度较高，其中遵化群体的多态性位点百分率最高，为97.18%，青龙和迁西群体次之，分别为95.77%和94.37%，表明遵化、迁西一带是燕山板栗资源遗传多样性最为丰富的区域。UPGMA聚类分析表明，迁西和宽城群体间的遗传相似性最大，亲缘关系最近；怀柔和邢台(太行山)群体间的遗传相似性最小，遗传差异较大。聚类分析图和主坐标分析图可将燕山板栗主产区青龙、迁西、宽城、兴隆、遵化和怀柔群体资源划为1类，而处于太行山区邢台群体为1类，说明燕山板栗和外来品种存在明显的差异，各群体的遗传关系和地理来源有一定关联性。 |
| 关键词: 燕山板栗 种质资源 遗传多样性 SSR |
| DOI：10.11841/j.issn.1007-4333.2020.04.07 |
| 投稿时间：2019-06-14 |
| 基金项目:河北省农林科学院青年基金(2018020106) |
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| Genetic diversity analysis of chestnut germplasm in Yanshan region based on SSR markers |
|
ZHANG Xinfang,ZHANG Shuhang,LI Ying,GUO Yan,WANG Guangpeng
|
| (Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, China) |
| Abstract: |
| To provide the basis for development and utilization of germplasm resources as well as genetic background analysis, the genetic diversity of 142 chestnut germplasm resources from Yanshan region and 9 cultivars collected from Taihangshan region were analyzed by 21 pairs selected SSR primers. A total of 71 alleles were successfully detected with these SSR primers ranged from 2 to 6 and with an average of 3. 38 alleles per locus. The polymorphic information content(PIC)ranged from 0. 614 5 to 0. 972 3, with an average value of 0. 866 8. The effective number of alleles(Ne), Nei's genetic diversity index(H), Shannon's information index(I), number of total genetic diversity index(Ht)and intra-population genetic diversity index(Hs)of eight populations were respectively 1. 457 8, 0. 309 9, 0. 468 0, 0. 306 2, 0. 291 2, indicating relatively high genetic diversity and variability of Yanshan chestnut populations. Among them, the polymorphic rate of Zunhua population was the highest, which accounted for 97. 18%, followed by Qinglong 95. 77% with and Qianxi 94. 37%. These results suggested that populations in Zunhua and Qianxi had the richest genetic diversity. UPGMA cluster analysis showed that Qianxi population and Kuancheng population had the closest genetic similarity and relationship, while the contrary was the case of Huairou population and Xingtai population. The clustering results and principal coordinate analysis showed the germplasm resources from Yanshan region were classified into one cluster, while those from Taihangshan region were classified into another, indicating that there was obvious difference between chestnuts originated from Yanshan and from Taihangshan and the genetic relationship largely correlated with the geographic origin. |
| Key words: Yanshan chestnut germplasm resources genetic diversity SSR |
