| 摘要: |
| 为探明番茄/黄瓜远缘嫁接不亲和时愈合口的形成过程，为后期砧穗间信息互作的研究奠定一定的理论基础，以黄瓜‘新泰密刺’、黑籽南瓜和番茄‘中杂9号’为试材，采用下胚轴斜靠接方法远缘嫁接番茄/黄瓜(接穗/砧木)，嫁接组合黄瓜/南瓜、黄瓜/黄瓜作为嫁接亲和性的对照。观察嫁接后第2、5、8和15天嫁接苗生长形态变化并统计成活率，对比分析黄瓜的近缘及远缘嫁接的嫁接口愈合过程。结果表明:1)嫁接接口处石蜡切片结果显示，对照黄瓜自嫁接和黄瓜与南瓜的嫁接均经历了相同的愈合过程，并最终成功地建立了形成层与输导组织，得以正常生长；2)分别用经木质部运输的品红溶液、经韧皮部运输的HPTS染料标记嫁接苗，结果显示远缘的番茄与黄瓜嫁接后，砧木与接穗虽然可以各自分化出愈伤组织，但二者愈伤组织的薄壁细胞无法真正地融合，机械性地对接在一起的木质部可以运输水分，使得番茄接穗不萎蔫，但韧皮部并未连通，无法进行其光合及同化产物运输、维持其生命活动，初步解释了远缘嫁接不亲和的解剖学过程。 |
| 关键词: 黄瓜 番茄 南瓜 远缘嫁接 嫁接口愈合 维管束连通 |
| DOI：10.11841/j.issn.1007-4333.2020.04.06 |
| 投稿时间：2019-06-25 |
| 基金项目:国家重点研发计划(2018YFD1000800)；国家自然科学基金(31872158)；国家大学生创新性实验计划(10080521) |
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| Histological anatomy analysis of inter-family grafting interface healing between tomato and cucumber |
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WU Ye,LIU Wenqian,HU Xi,GAO Lihong,ZHANG Wenna
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| (College of Harticulture/Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| The aims of this study were to explore the healing formation process of incompatible inter-family grafting between tomato and cucumber, and provide theoretical foundation for future research on interaction between rootstocks and scions. Three species, cucumber ‘Xintaimici'(Cucumis sativus L. ), figleaf gourd(Cucurbita ficifolia Bouché) and tomato ‘Zhongza No. 9'(Lycopersicon esculentum Mill. )were taken as study objects in this study. Inter-family grafting with tomato(scion)/cucumber(stock)was performed by using hypocotyl grafting method with homo-grafted cucumber/cucumber, inner-family hetero-grafted cucumber/figleaf gourd as controls. Firstly, the morphological changes of three graft combination seedlings were investigated at 2, 5, 8 and 15 day after grafting and the grafting survival rate was analyzed. Moreover, the anatomical changes of healing process between inter-family grafts, homo-grafts and inner-family hetero-grafts were compared and analyzed. The results indicated that: 1)By paraffin section of graft union detection, both homo-grafts cucumber/cucumber and inner-family hetero-grafted cucumber/figleaf gourd were undergoing same healing process, and the cambium and vasculature system were established successfully. While both parties of inter-family graft tomato/cucumber can also dedifferentiate callus respectively, however parenchyma cells of each callus were not able to fuse together veritably. 2)By labeling systems of fuchsin solution transported via xylem and HPTS dye transported via phloem respectively, it was found that the mechanically docked xylem of grafted seedlings could transport water and keep scion from wilting, but phloem was still not able to fuse. Therefore, most of nutrients such as photosynthetic assimilated products could not be transported from source to sink tissues to maintain vitality, which explained the preliminary anatomical mechanism of the incompatible of inter-family grafting. |
| Key words: cucumber tomato pumpkin inter-family grafting graft union healing vasculature connection |
