| 摘要: |
| 为探讨稀土壳糖胺螯合盐(RECC)对热应激黄羽肉鸡脂肪组织学及三磷酸结合盒转运体G1(ATP-binding cassette G1，ABCG1)mRNA表达的影响。随机选取90只饲养至29日龄黄羽肉鸡，分为3个处理组，即常温对照组、热应激组和RECC组(热应激+基础日粮中添加0.03% RECC)。分别在热应激处理1 和2 周后，采集肉鸡内脏、皮下及肌间脂肪，实时荧光定量(qRT-PCR)方法检测脂肪细胞大小变化及脂肪组织中三磷酸结合盒转运体G1 mRNA表达变化。结果表明:热应激1 周后，与对照组和热应激组相比，RECC组肉鸡内脏、皮下和肌间脂肪细胞直径与相比显著减小(P<0.01)，3 种脂肪组织中ABCG1 mRNA表达水平显著升高(P<0.05)；热应激 2周后，与对照组和热应激组相比，RECC组肉鸡内脏和肌间脂肪细胞直径显著减小(P<0.05)，皮下脂肪细胞直径无显著变化。ABCG1 mRNA表达水平的检测结果表明:与热应激组相比，RECC组内脏和皮下脂肪组织中ABCG1 mRNA表达水平显著降低(P<0.01)。综上所述，RECC可通过减小脂肪细胞直径和促进ABCG1的表达，从而抑制热应激1周肉鸡体内脂肪沉积。 |
| 关键词: 黄羽肉鸡 热应激 稀土壳糖胺螯合盐 脂肪组织 ABCG1 |
| DOI：10.11841/j.issn.1007-4333.2020.03.07 |
| 投稿时间：2019-09-18 |
| 基金项目:安徽省自然科学基金项目(1708085MC81)；安徽省重点研发项目(1704a07020069) |
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| Effects of rare earth chitosan chelate on adipose histology and ABCG1 mRNA expression in thermal stressed broilers |
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PENG Mengling,DONG Jing,LIU Qi,HU Wenye,ZHANG Suzi,WANG Juhua,ZHOU Jie
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| (College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China) |
| Abstract: |
| The aim of this study was to investigate the effects of rare earth chitosan chelate salt(RECC)on adipose histology and the expression levels of ATP-binding cassette G1(ABCG1)mRNA in yellow feather broilers treated with thermal stress. A total of 90 29-day-old yellow-feathered broilers were randomly divided into 3 treatment groups: Normal temperature control group, thermal stress group and RECC group(thermal stress + basal diet added 0. 03% RECC). The visceral, subcutaneous and intermuscular fats of broilers were collected after 1 and 2 weeks of thermal stress treatment. The size of adipocytes and the expression levels of ABCG1 mRNA in each adipose tissue were determined by qRT-PCR. The results showed that the diameter of adipocytes in visceral, subcutaneous and intermuscular was significantly reduced in the RECC group compared with the control group and the thermal stress group(P<0. 01)after 1 week treated with thermal stress, while the expression level of ABCG1 mRNA was significantly increased(P<0. 05). The diameter of adipocytes in visceral and intermuscular in RECC group was significantly decreased compared with control group and thermal stress group(P<0. 05)after 2 weeks treated with thermal stress; The expression levels of ABCG1 mRNA in visceral and subcutaneous adipose tissues in RECC group were significantly decreased compared with thermal stress group(P<0. 01). In summary, RECC could inhibit the fat deposition through decreasing the diameters of adipocytes and increasing the level of ABCG1 mRNA in broilers treated with thermal stress for 1 week. |
| Key words: yellow-feathered broiler rare earth chitosan chelate salt thermal stress adipocytes tissue ABCG1 |
