| 本文已被：浏览 850次 下载 394次 |
码上扫一扫！ |
| 围产前期奶牛血清相关能量平衡指标和脂肪因子对酮病的预警作用及意义 |
|
肖劲邦, 朱颍琨, 钱柏霖, 邓俊良, 彭广能, 余树民, 左之才, 马晓平, 钟志军, 任志华, 周紫峣, 刘海峰, 曹随忠, 沈留红
|
|
|
| (四川农业大学 动物医学院/动物疫病与人类健康四川省重点实验室/奶牛疾病研究中心, 成都 611130) |
|
| 摘要: |
| 为探究围产期奶牛产前14和7 d血清能量平衡指标以及脂肪因子对产后酮病的预警作用及意义。从奶牛产前21 d至产后21 d，每隔7 d跟踪采集60头围产期奶牛尾静脉血，最终筛选出健康奶牛21头，酮病奶牛17头，检测奶牛各时间点血清BHBA、NEFA、Glu、TG、INS、ADP、LEP、抵抗素、IL-6和TNF-α含量，利用二元Logistic回归模型筛选预警酮病的指标，再采用ROC曲线确定各风险评估指标的诊断效果及最佳分界值。结果表明：奶牛产前14 d时，血清BHBA和NEFA可作为酮病的预警指标；BHBA的最佳分界值为>0.42 mmol/L，对应的敏感度为58.8%，特异度为81.0%；NEFA的最佳分界值为>0.47 mmol/L，对应的敏感度为58.8%，特异度为90.5%；产前7 d时，血清BHBA和NEFA可作为酮病的预警指标，BHBA的最佳分界值为>0.34 mmol/L，对应的敏感度为76.5%，特异度为66.7%；NEFA的最佳分界值为>0.32 mmol/L，对应的敏感度为94.1%，特异度为47.6%。本研究发现产前14和7 d血清ADP、LEP、抵抗素、IL-6和TNF-α均不适合为产后酮病的预警指标。然而，随预产期临近，该5种脂肪因子Logistic曲线的显著性逐渐向具有统计学意义接近，存在作为酮病预警指标的潜力。 |
| 关键词: 奶牛 围产期 酮病 脂肪因子 ROC曲线 |
| DOI：10.11841/j.issn.1007-4333.2019.09.09 |
| 投稿时间：2019-01-03 |
| 基金项目:国家重点研发计划项目（2018YFD0500900）；四川省科技厅科技计划项目（2019YJ0650）；四川省科技厅科技创新苗子工程项目（2017086）；四川农业大学学科建设双支计划（03572174）；四川农业大学本科生科研兴趣培养项目（2019186） |
|
| Early warning function and significance of serum energy balance index and adipokine levels for ketosis in dairy cows during early prenatal |
|
XIAO Jinbang, ZHU Yingkun, QIAN Bailin, DENG Junliang, PENG Guangneng, YU Shumin, ZUO Zhicai, MA Xiaoping, ZHONG Zhijun, REN Zhihua, ZHOU Ziyao, LIU Haifeng, CAO Suizhong, SHEN Liuhong
|
| (College of Veterinary Medicine/Key Laboratory of Animal Disease and Human Health of Sichuan Province/The Medical Research Center for Cow Disease, Sichuan Agricultural University, Chengdu 611130, China) |
| Abstract: |
| In order to explore the early warning function and significance of serum energy balance index for ketosis in dairy cows on 14 and 7 d before delivery during early prenatal.A total of 60 prenatal dairy cows' tail venous blood were collected respectively at 7 days interval from 21 days before delivery to 21 days after delivery,and 21 healthy dairy cows and 17 ketosis cows were screened.The levels of BHBA,NEFA,GLU,TG,INS,ADP,LEP,resistin,IL-6 and TNF-α in serum were detected.The binary logistic regression model was used to screen the indexes that could alert ketosis and ROC curve was then used to determine the diagnostic effect and the optimal demarcation value of the risk assessment indexes.The results showed that:On 14 days before delivery,the levels of BHBA and NEFA in serum could be used as early warning indicators for ketosis.The optimal threshold value of BHBA was >0.42 mmol/L and its corresponding sensitivity was 58.8% and specificity was 81.0%.The optimal threshold value of NEFA was >0.47 mmol/L,its corresponding sensitivity is 58.8% and specificity is 90.5%;On 7 days before delivery,the levels of BHBA and NEFA in serum could be used as early warning indicators for ketosis.The optimal threshold value of BHBA was > 0.34 mmol/L,its corresponding sensitivity was 76.6% and specificity was 66.7%.The optimal threshold value of NEFA was > 0.32 mmol/L,corresponding sensitivity was 94.1% and specificity is 47.6%.However,the levels of ADP,LEP,resistin,IL-6 and TNF-α in serum of dairy cows on 14 and 7 days before delivery were found not suitable for the early warning for ketosis.But when the time approaching of the expected delivery period,the significance of logistic curves of the five adipokines gradually displayed statistical significances,indicating that they might be potential early warning indexes. |
| Key words: dairy cow prenatal period ketosis adipokine ROC curve |
