| 摘要: |
| 为论证单一固态Bacillus velezensis 157发酵豆粕中混合碱处理玉米秸秆能否有利于提高内切葡聚糖酶、淀粉酶、木聚糖酶和果胶酶的产酶量，利用DNS法对碱处理玉米秸秆与豆粕混合添加质量比进行优化后，采用单因素法分别对单一发酵豆粕、碱处理玉米秸秆以及混合发酵碱处理玉米秸秆-豆粕中底物与水分质量比、发酵温度以及发酵时间进行优化，并对发酵过程中B.velezensis 157的生长曲线进行测定。结果表明：经发酵条件优化后，以碱处理玉米秸秆和豆粕的质量比为1.0：1.0，底物与水分质量比为1.0：0.5；B.velezensis 157于37℃培养温度下发酵24 h后，混合发酵组的内切葡聚糖酶、淀粉酶、木聚糖酶、果胶酶以及菌落数，均高于单一发酵碱处理玉米秸秆组和豆粕组，酶活力分别可达56.83±1.47、1 949.28±14.41、16.52±0.79和14.53±0.56 U/g，相应酶活力与碱处理玉米秸秆组相比，分别提高了7.81、6.41、1.23和2.53倍；与豆粕组相比，分别提高了1.17、1.35、1.07和1.21倍；同时，混合发酵组中B.velezensis 157菌落数高于豆粕组和碱处理秸秆组。因此，B.velezensis 157混合固态发酵有利于提高B.velezensis 157内切葡聚糖酶、木聚糖酶、淀粉酶和果胶酶的酶产量，节约了时间和成本，为动物饲料酶制剂和生物饲料的开发提供依据。 |
| 关键词: Bacillus velezensis 157 固态发酵 产酶 发酵条件优化 |
| DOI：10.11841/j.issn.1007-4333.2019.09.08 |
| 投稿时间：2018-12-28 |
| 基金项目:吉林省科技厅重点科技研发项目（20180201042NY）；吉林省农业科学院创新工程项目（CXGC2017JQ003）；安全绿色饲料添加剂中试中心项目（20160626002NY） |
|
| Optimization of fermentation conditions for the production of various lignocellulases by Bacillus velezensis 157 under solid-state fermentation |
|
CHEN Long1, WU Xingli1, YU Wei1, GU Wei2, XU Haiyan2, QIAN Aidong3, WANG Chunfeng3, ZHANG Fangyu1
|
| (1.Branch of Animal Husbandry, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China;2.Shandong BaoLai-LeeLai Bioengineering Co., Ltd., Tai'an 271000, China;3.College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China) |
| Abstract: |
| The purpose of this study was to evaluate whether the mixture of alkali-treated maize straw in soybean meal could improve the production of cellulase,amylase,xylanase and pectinase by Bacillus velezensis 157 under solid-state fermentation.The mixture ratio of alkali-treated maize straw and soybean meal were optimized by using DNS method.The ratio of substrate to water,fermentation temperature and time were optimized by the method of single factor among single fermentation soybean meal,alkali-treated maize straw and mixed fermentation of alkali-treated maize straw and soybean meal,respectively.The growth curve of B.velezensis 157 during fermentation was also determined.The results showed that:After optimization of fermentation conditions,alkali-treated maize straw and soybean meal were treated as substrate in a ratio of 1.0:1.0,and the ratio of substrates to moisture is 1.0:0.5.The cellulase,amylase,xylanase,pectinase and colony number in the mixed fermentation group by B.velezensis 157 was higher than that the single fermentation of the alkali-treated maize straw and soybean meal at 37℃ and 24 h,and the activities of cellulase,amylase,xylanase,pectinase in mixture fermentation are 56.83±1.47,1 949.28±14.41,16.52±0.79 and 14.53±0.56 U/g,respectively.The corresponding enzyme activity of mixed fermentation group was 7.81,6.41,1.23 and 2.53 times higher than that of alkali-treated maize straw group,and 1.17,1.35,1.07 and 1.21 times than the soybean meal group,respectively.Meanwhile,the number of B.velezensis 157 colony in mixed fermentation group was higher than both of them.In conclusion,it can be seen that the mixture of alkali-treated maize straw and soybean meal under solid-state fermentation at certain conditions improved the yield of cellulase,amylase,xylanase and pectinase produced by B.velezensis 157.This method saved time and reduced cost.This study laid a foundation for the research and development of animal feed additives and biological feed. |
| Key words: Bacillus velezensis 157 solid-state fermentation enzyme production fermentation condition optimization |
