| 本文已被：浏览 1490次 下载 802次 |
码上扫一扫！ |
| 玉米高油型诱导系CHOI1和CHOI3的单倍体鉴定效率评价 |
|
董昕1,2, 田小龙1, 李伟1, 刘晨旭1, 杨华2, 官玲3, 李淑君2, 张丕辉2, 陈绍江1
|
|
|
| (1.中国农业大学 国家玉米改良中心, 北京 100193;2.重庆市农业科学院 玉米研究所, 重庆 401329;3.重庆市农业科学院 农业生物技术研究中心, 重庆 401329) |
|
| 摘要: |
| 为系统评价新型高油型诱导系油份鉴定单倍体的效率，以籽粒油分含量分别为8.71%和8.72%的2个高油型诱导系CHOI1和CHOI3为父本，分别在海南省和北京市对10个普通玉米杂交种进行诱导，利用油分和R1-nj遗传标记对诱导产生的籽粒分别进行单倍体的鉴定，并对2种鉴定方法进行评价。结果表明：与遗传标记法相比，油分标记法的单倍体鉴定准确率更高，且不易受环境影响。CHOI1和CHOI3诱导杂交籽粒与其母本单倍体籽粒间的平均油分均值差异分别为2.21%和2.02%，变异范围分别为1.86%~2.63%，1.54%~2.46%；各组合诱导率平均值分别为8.30%和8.59%，变异范围分别为6.25%~10.40%，5.81%~11.19%。诱导系CHOI1作父本时，以油份均值（μ）+1.645×标准差（σ）作为区分单倍体的阈值，所测组合的单倍体鉴定准确率均高于90%，漏选率均低于10%。以4.0%的籽粒油分含量为通用阈值，所测组合均可达到90%以上的鉴定准确率。 |
| 关键词: 玉米 单倍体 油分 R1-nj遗传标记 高油型诱导系 |
| DOI：10.11841/j.issn.1007-4333.2019.06.01 |
| 投稿时间：2018-06-11 |
| 基金项目:国家重点研发计划（2016YFD0101201）；国家玉米产业技术体系（CARS-02-04）；重庆市农业科学院青年创新团队项目（Nky-2018QC02）；重庆市基本科研业务（2016cstc-jbky-00506）；重庆市科委项目（cstc2016shms-ztzx80013） |
|
| Evaluation the efficiency of maize haploid identification with High-Oil Inducers CHOI1 and CHOI3 |
|
DONG Xin1,2, TIAN Xiaolong1, LI Wei1, LIU Chenxu1, YANG Hua2, GUAN Ling3, LI Shujun2, ZHANG Pihui2, CHEN Shaojiang1
|
| (1.National Maize Improvement Center of China, China Agricultural University, Beijing 100193, China;2.Maize Research Institute, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China;3.Agricultural Biotechnology Research Center, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China) |
| Abstract: |
| The objective of this study was to systematically evaluate the efficiency of haploid identification by oil content (OC) with new High-Oil Inducers.Ten hybrids were induced by two High-Oil Inducers CHOI1 and CHOI3 with OC 8.71% and 8.72% respectively in Beijing and Hainan.The haploid identification by OC and R1-nj markers were evaluated and compared.The results showed that the accuracy rate (AR) of OC system,which was not influenced by environments,was higher than that of R1-nj system.When OC system was used to identify haploids induced by CHOI1 and CHOI3,the difference in the mean OC of haploids and crossing seeds were respectively 2.21% and 2.02% and the ranges of variation were 1.86%-2.63% and 1.54%-2.46%,respectively.The haploid induction rates were 8.30% and 8.59%,with ranges of 6.25%-10.40% and 5.81%-11.19%,respectively.When indexes mean (μ) +1.645×standard deviation (σ) was used as the oil content threshold value to classify the haploids induced by CHOI1,the ARs were all beyond 90%.In addition,the missing rates were all below 10%.The ARs of all the crosses were more than 90% by using 4% seed oil as the general threshold. |
| Key words: maize haploid oil content R1-nj marker High-Oil Inducer |
