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松嫩盐碱草地蒲公英根围AM真菌侵染特性及种类多样性
高璿濛, 王玉丹, 杨春雪
0
(东北林业大学 园林学院, 哈尔滨 150040)
摘要:
为深入了解松嫩盐碱草地蒲公英根围AM真菌类型,丰富该地AM真菌资源多样性,以及为更高效地修复盐碱农业用地提供新的途径及想法,利用形态学鉴定的研究方法,对松嫩盐碱草地蒲公英(Taraxacum mongolicum)根围AM真菌(Arbuscular mycorrhizae fungi)侵染特性及其种类多样性进行研究。结果表明:1)在pH不同的土壤生态环境下,蒲公英根系均与AM真菌形成共生关系,并形成典型的重楼型(Paris-type,P型)菌根结构,且侵染率均达到100%。2)对利用湿筛法提取出的蒲公英根围土壤AM真菌孢子进行形态学鉴定,共鉴定出6属21种,其中球囊霉属(Glomus)10种,为本区域蒲公英根围AM真菌优势属,摩西球囊霉占比最多,为本区域蒲公英根围优势AM真菌;无梗囊霉属(Acaulospora)5种;盾巨孢囊霉属(Scutellospora)3种;和平囊霉属(Pacispora)、原囊霉属(Archaeospor)、巨孢囊霉属(Gigaspora)各1种。3)由于此次试验中侵染率极高,说明轻度碱性的土壤情况可能有利于AM真菌与宿主植物亲和以及AM真菌的生长,并且该样地处于适宜菌丝生长的pH范围内。
关键词:  盐碱草地  AM真菌  蒲公英  多样性
DOI:10.11841/j.issn.1007-4333.2019.05.11
投稿时间:2018-02-08
基金项目:国家自然科学基金青年科学基金项目(31601986);中央高校基本科研业务费专项资金资助(2572018BK02);东北油田盐碱植被恢复与重建教育部重点试验室开放基金(SAVER1608);黑龙江省博士后科研启动金(LBH-Q16005)
Mycorrhizal fungal infection characteristics and the diversity of Taraxacum mongolicum rhizosphere in songnen saline-alkaline grassland
GAO Xuanmeng, WANG Yudan, YANG Chunxue
(College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China)
Abstract:
To further understand the AM fungi types in songnen saline-alkaline grassland of dandelion (Taraxacum mongolicum) root circumstance,rich the diversity of AM fungi resources,as well as provide new approaches and ideas for more efficient ecological remediation of saline-alkaline agricultural land,the morphological identification method was used to explore the infection characteristics and diversity of AM fungi.The results showed that:1) In the soil ecological environment with different pH,the dandelion root system was symbiotic with AM fungi.A typical paris-type (p type) mycorrhizal structure was formed,and the colonization rate reached 100%.2) Morphological identification of the AM fungi spores in the surrounding soil of dandelion root extracted by wet screening method was carried out.A total of 21 species of 6 genera were identified,including 10 species from Glomus,the dominant genus in the root of dandelion in this region.There were Acaulospora (5 species),Scutellospora (3 species),and Pacific spora,Archaeospor and Gigaspora (1 species each).3) The high infection rate in this experiment indicated that the mildly alkaline soil was conducive to the affinity between AM fungi and host plants as well as the growth of AM fungi,and the pH range of the site was suitable for mycelium growth.
Key words:  saline-alkaline grassland  AM fungi  Taraxacum mongolicum  diversity