| 摘要: |
| 为提高芍药属远缘杂交结实率，对3类5个远缘杂交组合进行柱头处理，即：1）牡丹组革质花盘亚组内杂交：‘凤丹白’ב芳纪’，紫斑牡丹ב雪映桃花’；2）革质花盘亚组和肉质花盘亚组杂交：紫牡丹ב日月锦’，卵叶牡丹×黄牡丹；3）牡丹组和芍药组间杂交：紫斑牡丹ב红艳争辉’。各组合采用4种处理方式：2.0%，3.5%，5.0% KCL溶液处理柱头；1.5%，3.0%，4.5% NaCl溶液处理柱头；25、50和75 mg/L GA3处理柱头；花粉培养液处理柱头，比较不同授粉方式对结实率的影响。结果表明：1）KCL溶液效果优于NaCl溶液，3.5% KCl效果最好；2）所有组合结实率最高值均出现在GA3处理中，GA3 25 mg/L最为稳妥；3）最佳培养液配方为蔗糖100 g/L+H3BO30.08 g/L+CaCl220 mg/L，但花粉培养液处理仅对革质花盘亚组内杂交组合‘凤丹白’ב芳纪’组合有促进作用。 |
| 关键词: 芍药属 远缘杂交 柱头处理 结实率 |
| DOI：10.11841/j.issn.1007-4333.2019.01.06 |
| 投稿时间：2018-03-02 |
| 基金项目:风景园林学科一流学科建设（000-111801001） |
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| Effects of four stigma treatments on the seed-setting rate of Paeonia distant hybridization |
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HAO Jinli, DONG Xiaoxiao, YUAN Tao
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| (School of Landscape Architecture/Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding/National Engineering Research Center for Floriculture/Beijing Laboratory of Urban and Rural Ecological Environment/Beijing Laboratory of urban and rural ecological environment, Beijing Forestry University, Beijing 10083, China) |
| Abstract: |
| To enhance the compatibility of distant hybridization for improving breeding efficiency, 5 cross combinations of 3 types were set:1) Leathery flower disc subgroups hybridization:Paeonia ostii ‘Fengdan Bai’×P. suffruticosa ‘Hoki’, P. rockii×P. suffruticosa ‘XueyingTaohua’; 2) Intersubsectional hybridization:P. delavayi×P. suffruticosa ‘JitsugetsuNishiki’, P. qiui×P. lutea; 3) Intersectional hybridization:P. rockii×P. lactiflora ‘HongyanZhenghui’. Each combination was processed in four treatments:KCl solution treatment (2.0%, 3.5% and 5.0% KC, respectively), NaCl solution treatment (1.5%, 3.0% and 4.5% NaCl respectively), GA3 treatment (25, 50 and 75 mg/L GA3, respectively). The stigmas were treated with these pollen culture solutions to compare their effects on seed-setting rate. The result showed that:1) The effect of KCl solution treatment was better than that of NaCl solution, and the concentration of 3.5% KCl worked the best; 2) The seed-setting rate in each combination all reached the highest in the GA3 treatments, and the 25 mg/L GA3 treatment was the most appropriate; 3) The optimum pollen culture solution was:Sucrose 100 g/L + H3BO3 0.08 g/L+CaCl2 10 mg/L, but it was only effective for Paeonia ostii ‘Fengdan Bai’ ×P. suffruticosa Hoki cross combination. |
| Key words: Paeonia distant hybridization treatment of stigma seed-setting rate |
