| 摘要: |
| 为揭示棉花植株体内赤霉素合成途径关键酶基因表达量与不同组织及环境条件的关系，以"中棉所49"（CCRI49）和"高脚棉"（396289）为材料，利用实时荧光定量PCR方法中的相对定量，以UBQ7为内参基因，对新疆维吾尔自治区阿拉尔和河南省安阳，2种不同生态环境下的CCRI49和396289不同组织内赤霉素合成途径相关基因的表达量进行检测。结果显示，在阿拉尔地区生长条件下，CCRI49果枝始生节位相比于安阳地区平均降低2.0，高脚棉平均降低2.6。在安阳地区，CCRI49和396289中赤霉素合成途径各关键酶基因在不同组织内的表达趋势基本一致，茎组织中各相关基因表达量高，为植株生长提供充足的活性赤霉素；在新疆阿拉尔地区，CCRI49和396289主要表现为赤霉素合成早期阶段CPS1、KS和KO等基因的表达量下调，GA2ox1表达量上调，以减少活性赤霉素的合成。结果表明，棉花植株赤霉素合成代谢途径关键酶基因的表达量发生改变，从而调控植株的生长发育来适应新疆干旱和盐渍化的生长环境。 |
| 关键词: 棉花 赤霉素 环境条件 基因表达量 |
| DOI：10.11841/j.issn.1007-4333.2019.01.02 |
| 投稿时间：2017-12-06 |
| 基金项目:国家重点研发计划（2018YFD0100300）；中央级公益性科研院所基本科研业务费专项（161012018019）；棉花生物学国家重点实验室自主课题（CB2017C14） |
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| Differential expression of the key enzyme of gibberellin biosynthesis pathway in cotton under different ecological conditions |
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SHI Jianbin, WANG Ning, ZHOU Hong, XU Qinghua, QIAO Wenqing, YAN Gentu
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| (Institute of Cotton Research/State Key Laboratory of Cotton Biology, Chinese Academy of Agricultural Sciences, Anyang 455000, China) |
| Abstract: |
| To explore the relationship between the key enzyme gene expression of gibberellin synthesis pathway and different tissues or environmental condition, cotton CCRI49 and 396289 were selected as material, and UBQ7 was selected as reference gene, Relative quantification real-time PCR was developed to determine the gibberellin key gene expression of different cotton organizations under two kinds of ecological environment. The results showed that:compared with Anyang, the average node of first fruiting/sympodial branch of CCRI49 and 396289 were repectively decreased by 2.0 and 2.6 under the growth condition of Alar. In Anyang region, the expression trends of gibberellin related gene were basically identical in tissues, but the expression level in stem was higher, which was beneficial for the growth of plants. In Alar region, the CPS1, KS, and KO gene expressions were significantly down-regulated in CCRI49 and 396289, while GA2ox1 gene expression was up-regulated. The down-regulation of CPS1, KS and KO reduced the synthesis rate of gibberellin, and the up-regulation of GA2ox1 decomposed the active gibberellin. To adapt to the arid and salinized growth environment of Xinjiang, the cotton plant regulate its growth and development by changing the expression of the key enzyme gene of gibberellin in the plant. |
| Key words: cotton gibberellin environmental condition gene expression |
