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| 利用SLAF-seq技术开发十倍体长穗偃麦草专化分子标记 |
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杨国堂1,2, 罗巧玲1,2, 刘利勤1, 张静1, 李振声1, 郑琪1
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| (1.中国科学院 遗传与发育生物学研究所/植物细胞与染色体工程国家重点实验室, 北京 100101;2.中国科学院大学, 北京 100049) |
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| 摘要: |
| 为开发长穗偃麦草（Thinopyrum ponticum）分子标记，以小偃68（西农）为试验材料，利用SLAF-seq技术获得9 667个小偃68外源染色体特异标签，筛选出823个长穗偃麦草专化分子标记。选取其中100个标记扩增20份小偃麦易位系，发现染色体构成相似的易位系具有相同的扩增结果，并得到小偃麦易位系的特异分子标记。应用这些标记可以快速检测长穗偃麦草遗传物质。 |
| 关键词: 长穗偃麦草 远缘杂交 SLAF-seq 分子标记 |
| DOI：10.11841/j.issn.1007-4333.2019.01.01 |
| 投稿时间：2018-02-09 |
| 基金项目:国家重点研发计划项目资助（2016YFD0102000） |
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| Development of Thinopyrum ponticum specific molecular markers by using SLAF-seq technology |
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YANG Guotang1,2, LUO Qiaoling1,2, LIU Liqin1, ZHANG Jing1, LI Zhensheng1, ZHENG Qi1
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| (1.State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, China;2.University of Chinese Academy of Sciences, Beijing 100049, China) |
| Abstract: |
| In order to develop markers of Thinopyrum ponticum, Xiaoyan 68 (XN) was taken as study material. A total of 9 667 specific fragments from the alien chromosomes of Xiaoyan 68 (XN) were screened by specific-locus amplified fragment sequencing (SLAF-seq) technology and 823 alien chromosome specific markers were developed. The results of PCR amplification analysis and the chromosome composition in wheat-Thinopyrum translocations showed that the lines amplified the same molecular markers carried the similar exogenous chromosome fragments. Specific markers of wheat-Thinopyrum translocations were obtained. All these newly developed specific markers could be used for rapid detection of the exogenous chromosome fragments in wheat-Th. ponticum progenies. |
| Key words: Thinopyrum ponticum wide hybridization SLAF-seq molecular markers |
