| 摘要: |
| 为探讨蜂王浆主蛋白（MRJPs）对四氯化碳（CCl4）引起小鼠急性肝损伤的保护作用，建立CCl4诱导的小鼠急性肝损伤模型，将模型小鼠随机分组，每组8只，空白对照组、模型组、药物对照组（联苯双酯）以及MRJPs低、中、高剂量组），检测各组小鼠血清中谷丙转氨酶（AST）、谷草转氨酶（ALT）等指标的变化，测定肝组织中丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性的变化，利用光镜观察肝组织病理形态学变化并测定肝脏中肿瘤坏死因子-α的基因表达量。结果表明：预先灌胃给予MRJPs的小鼠，可以显著抑制CCl4诱导的血清ALT和AST活性水平（P<0.01）；MRJPs可以显著抑制MDA的生成（P<0.01），从而抑制肝脏脂质过氧化；MRJPs可以显著抑制CCl4诱导的小鼠肝脏SOD活性的降低（P<0.01），从而增强小鼠肝脏的抗氧化能力；组织病理学检测结果也表明，MRJPs可有效抑制CCl4诱导的急性肝损伤；MRJPs诱导CCl4诱导TNF-α基因水平增加（P<0.05），从而调控免疫系统。综上，蜂王浆主蛋白对CCl4引起的小鼠急性肝损伤具有保护作用。 |
| 关键词: 小鼠 蜂王浆主蛋白 肝损伤 四氯化碳 |
| DOI：10.11841/j.issn.1007-4333.2018.11.10 |
| 投稿时间：2018-02-16 |
| 基金项目:国家自然科学基金项目（31660633）；江西省科技支撑计划（20121112040007） |
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| Protective effect of MRJPs on carbon tetrachloride-induced acute liver injury in mice |
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MENG Chao1,2, ZHENG Shuangyan1, KANG Lumei1, CHENG Wei1, FANG Jin1, XU Baohua1
|
| (1.Experimental Animal Science Center, Nanchang University, Nanchang 330006, China;2.Pingyi County People's Hospital, Pingyi 273300, China) |
| Abstract: |
| In order to explore the effects of MRJPs (Major royal jelly proteins), the effects of the main water-soluble component of RJ on carbon tetrachloride (CCl4)-induced liver injury were investigated. The CCl4-induced acute liver injury model was established. Mice were randomly divided into six groups including negative control, positive control (CCl4 treated control group), Drug control, MRJPs (480 mg/kg) plus CCl4 treated group, MRJPs (320 mg/kg) plus CCl4 treated group and MRJPs (160 mg/kg) plus CCl4 treated group, respectively. The mice were pre-treated orally with MRJPs (480, 320 or 160 mg/kg) once daily for 28 days before CCl4 (10 mL/kg of 0.2% CCl4 solution in olive oil) injection. The hepato-protective effect of MRJPs was evaluated by the biochemical parameters related to hepatic damages, such as ALT, AST, SOD, MDA and TNF- α. The results showed that:MRJPs inhibited serum ALT and AST activities compared with positive group; MRJPs remarkably increased the levels of SOD and decreased MDA in liver; Histopathological test results showed that MRJPs effectively inhibited the acute liver injury induced by CCl4. Further investigation demonstrated that MRJPs markedly increased the gene expression of TNF- α in liver. In conclusion, MRJPs protected mouse from CCl4-induced acute liver injury. |
| Key words: mice major royal jelly proteins the hepatoprotective effect CCl4 |
