| 摘要: |
| 为探讨饲料添加剂对球虫感染肉鸡早期肠道健康的影响，选取400只1日龄健康AA肉公鸡为研究对象，随机分为4组各5个重复，每个重复10羽。4组分别为基础日粮组、基础日粮+0.08%抗生素组、基础日粮+0.05%益生菌组及基础日粮+1%绿茶粉组。每组肉鸡14日龄时分别灌喂1 mL （5×104）柔嫩艾美尔球虫和生理盐水。每2周空腹称重1次。分别于球虫侵染后的第4、7、10天收集粪便，用于球虫卵计数。于19、24日龄每组的5个重复中各取1只，共屠宰40只鸡，检测各肠段长度和重量，并截取其2 cm十二指肠、空肠、回肠和盲肠用于制作切片。结果表明：球虫侵染第10天时，抗生素组和益生菌组的虫卵数显著低于对照组（P<0.05），绿茶粉组球虫虫卵数低于对照组，但差异不显著（P>0.05）；饲料中添加1%绿茶粉降低肉鸡早期（0~14 d）增重（P<0.05），肉鸡第15~28天增重在各饲料添加剂组间无显著差异；益生菌组回肠长度显著高于其它各组（P<0.05），回肠壁厚显著低于对照组与绿茶粉组（P<0.05），与抗生素组差异不显著；绿茶粉组回肠壁厚、绒毛长度、隐窝深度、绒隐比和盲肠壁厚与各组间均无显著性差异。综上，球虫感染影响肉鸡肠道发育，导致空肠发育缓慢，盲肠壁增厚。绿茶粉可以缓解因球虫感染导致的体重下降，但对肉鸡早期（0~14 d）生长发育无显著促进作用。益生菌不影响肉鸡15~28日龄增重，并减少球虫感染后粪便中的虫卵数。 |
| 关键词: AA肉鸡 球虫 绿茶粉 肠道健康 |
| DOI：10.11841/j.issn.1007-4333.2018.11.08 |
| 投稿时间：2018-02-12 |
| 基金项目:农业部茶产业技术体系项目（nycytx-26）；安徽省领军人才团队项目 |
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| Effects of feed additives on the intestinal health of broilers during the early period of coccidial infection |
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LIU Xue1, DU Yeye1, LI Tao2, GENG Zhaoyu1, CHEN Xingyong1, WAN Xiaochun2
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| (1.College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;2.Key Laboratory of Tea Biochemistry and Biotechnology of Ministry of Agriculture and Ministry of Education, Anhui Agricultural University, Hefei 230036, China) |
| Abstract: |
| To investigate the effects of feed additives on the early intestinal health of broiler chickens affected by coccidiosis, a total of 400 healthy 1-day-old AA broilers were randomly divided into 4 treatment groups consisting of 5 replicates with 10 in each. Four treatments were basal diet, basal diet + 0.08% antibiotics, basal diet + 0.05% probiotics and basal diet + 1% green tea powder. At 14 days old, each group was intragastrically administered with1 mL (5×104) Eimeria tenella and saline. The broiler's body weight was recorded every two weeks after 12 h fasting. Their feces were collected on day 4, 7 and 10 d after coccidial challenge for egg counting. One broiler from each replicate was sampled at the age of 19 and 25 d for intestine measurement, respectively. A total of 40 chickens were taken. and 2 cm of intestine was collected and fixed in 10% formalin. The egg number of broilers fed with diet containing antibiotics or probiotics was significantly lower than those fed with basal diet P<0.05 10 days after coccidial challenge. The egg number of broilers fed with diet containing green tea powder was significantly lower than those fed with basal diet (P>0.05). The broilers fed with diet containing green tea powder exhibited significant lower body weight at 14 d than those fed with basal diet or antibiotic supplementation (P<0.05). However, the body weight exhibited no significant difference among different groups of broilers at 28 d. The length of ileum of broilers fed diet containing probiotics was longer than those in other groups (P<0.05). And the thickness of ileum was significantly lower than those fed with basal diet or diet containing green tea powder. In conclusion, coccidial infection led to slower growth of jejunum and thickened cecum. The green tea powder addition alleviated the body weight loss caused by coccidiosis while exhibited no effect on the early growth promotion of broiler before the age of 14 days. The probiotics did not affect the weight gain of broilers from 15 to 28 days of age and reduced the number of eggs in feces after coccidia infection. |
| Key words: AA broiler coccidiosis green tea powder intestinal health |
