| 本文已被：浏览 1212次 下载 503次 |
码上扫一扫！ |
| 产纤维素酶菌株Trametes sp.LYW-1的分离鉴定及产酶条件分析 |
|
关怡1, 尹娣1, 杜茜1, 沙艺琛2, 赖姗姗2, 赵超2,3
|
|
|
| (1.福州大学 福建省海洋酶工程重点实验室, 福州 350108;2.福建农林大学 食品科学学院, 福州 350002;3.福建省海洋藻类活性物质制备与功能开发重点实验室, 福建 泉州 362000) |
|
| 摘要: |
| 为寻找新纤维素酶和纤维素酶基因资源，以羧甲基纤维素钠作为唯一碳源，从湿润木屑中分离和筛选获得1株高产纤维素酶目的菌株LYW-1。对该菌保守序列ITS进行扩增和测序后，提交NCBI并与相近物种ITS序列进行比对和分析，基于亲缘关系构建系统发育树。将ITS rDNA保守序列比较分析结果与形态学观察结果相结合，初步鉴定该菌株属于栓菌属（Trametes）。以羧甲基纤维素钠溶液为底物，采用3’5’-二硝基水杨酸（DNS）法测定纤维素酶活力，分析pH、温度及培养基中同碳源、氮源对该菌产纤维素酶活力的影响。结果表明，菌株LYW-1在pH 7.0、37℃、淀粉为碳源和乙酸铵为氮源时为最适培养条件，总酶活力达到最大。 |
| 关键词: 栓菌属 纤维素酶 酶活力 产酶条件 |
| DOI：10.11841/j.issn.1007-4333.2018.10.12 |
| 投稿时间：2017-09-19 |
| 基金项目:福建省教育厅中青年教师教育科研项目（JAT170078）；国家自然科学基金（31701844）；福建省福州市科技计划项目（2017-G-83、2017-N-36）；福州大学校科研启动基金（XRC-1661）；福建省海洋藻类活性物质制备与功能开发重点实验室开放性课题（2017FXSK05）；农业部闽台作物有害生物综合治理重点实验室开放研究基金，闽台作物有害生物生态防控国家重点实验室开放基金资助 |
|
| Isolation and identification of cellulase-producing Trametes sp. LYW-1 and its enzyme-producing conditions |
|
GUAN Yi1, YIN Di1, DU Xi1, SHA Yichen2, LAI Shanshan2, ZHAO Chao2,3
|
| (1.Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou, 350116 China;2.College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002 China;3.Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou 362000 China) |
| Abstract: |
| To find new cellulase-producing microbial resource, fungus LYW-1 was isolated from wet wood chips by selection medium with CMC (Carboxyl methyl cellulose) as sole carbon source. Conserved ITS (Internal transcribed spacer) sequence of LYW-1 was amplified from genome and sequenced at Invitrogen. The verified ITS sequence was subjected to online BLAST analysis with other selected fungi. Phylogenetic analysis was then performed by MEGA 4.0. Combining the results of molecular analysis with morphological observation, LYW-1 was identified as a fungus belongs to Trametes. The cellulose activity of LYW-1 was assayed by 3',5'-dinitrosalicylic acid (DNS) method. The effect of different pH, temperature and carbon or nitrogen source on enzyme activity was assayed. The result showed that enzyme activity reached maximum when the fungus was cultured in fluid medium by using starch as carbon source and ammonium acetate as nitrogen source. The optimal pH and temperature for enzyme producing was pH 7.0 and 37℃, respectively. |
| Key words: Trametes cellulase enzyme activity condition of cellulase production |
