| 摘要: |
| 为获得马鹿（Cervus elaphus）Ghrelin全长cDNA序列并进行序列比较和分析，本试验以马鹿皱胃黏膜组织内提取的总RNA为模板，通过RT-PCR、RACE和基因克隆等技术进行克隆。结果表明：获得长度为539 bp的马鹿cDNA全序列（GenBank accession no.KX857494），其中包括46 bp的5'非编码区（5'UTR），351 bp的开放阅读框（ORF），编码116个氨基酸残基的前原Ghrelin（preproghrelin），128 bp的3'非编码区（3'UTR）和poly（A）14；Ghrelin的氨基酸同源性分析表明，马鹿Ghrelin cDNA全序列与驯鹿、梅花鹿、山羊、绵羊、牛等物种间的同源性较高，进化树分析与其亲缘关系远近一致。 |
| 关键词: 马鹿 Ghrelin cDNA 克隆 序列分析 |
| DOI：10.11841/j.issn.1007-4333.2018.10.11 |
| 投稿时间：2017-12-15 |
| 基金项目:内蒙古自然科学基金项目（2013MS0408） |
|
| Cloning and sequence analysis of the full-length Ghrelin cDNA in Cervus elaphus |
|
ZHANG Man, JIN Xin, WANG Yunhe, WEI Fang, WEN Jingyi, ZHANG Zhaoyi, YANG Yinfeng
|
| (College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China) |
| Abstract: |
| In order to obtain the full-length cDNA sequences of Ghrelin Cervus elaphus in total RNA was extracted from abomasum mucosa and the cDNA was obtained by RT-PCR, RACE and cloning technology. The results indicated that:A 539 bp (GenBank accession no. KX857494) amplicon of Cervus elaphus Ghrelin cDNA was cloned successfully. It was consisted by a 46 bp 5' UTR, 351 bp ORF encoding a preproghrelin of 116 amino acids, 128 bp 3' UTR and ploy(A)14. The amino acid homology analysis of Ghrelin showed that the full-length Ghrelin cDNA in Cervus elaphus was highly homologous to the reindeer, Cervus nippon, goat, sheep and cattle, and the phylogenetic tree analysis is consistent with its genetic relationship. This study provided basis for further analysis of the expression and regulation of Ghrelin gene in Cervus elaphus. |
| Key words: Cervus elaphus Ghrelin cDNA clone sequence analysis |
