| 摘要: |
| 为研究饲粮中添加硬脂酰辅酶A去饱和酶（SCD）的抑制剂（梧桐籽油）和促进剂（罗格列酮）对育肥绵羊背最长肌及皮下脂肪中脂代谢关键调节因子和相关酶mRNA表达量的影响，选用18只平均体重为27.71±2.64 kg、生理状况相似的杂交公羊（美利奴♂×小尾寒羊♀）随机分为3组，每组6只，进行单栏饲养（2.0 m长×1.2 m宽）。对照组（C组）饲喂含4.8%胡麻籽的饲粮，梧桐籽油组（W组）饲喂对照组饲粮+15 g/d梧桐籽油，罗格列酮组（L组）饲喂对照组饲粮+8 mg/d罗格列酮。试验期50 d，其中过渡期10 d，预饲期5 d，正式试验期为35 d。结果表明：1）与对照组相比，W组背最长肌和皮下脂肪中PPAR-γ mRNA表达量分别上调了42%和28%，背最长肌中SREBP-1和SCD mRNA表达量分别下降了20%和24%（P<0.05）；2）与对照组相比，L组背最长肌中PPAR-γ和SREBP-1 mRNA表达量均有不同程度的提高，分别提高了93%和35%（P<0.05），FAS、LPL和SCD mRNA表达量分别提高了36%、56%和22%（P<0.05），L组皮下脂肪中PPAR-γ和FAS mRNA的表达量分别提高了25%和32%（P<0.05）。研究表明梧桐籽油可以提高背最长肌及皮下脂肪中PPAR-γ mRNA的表达量，降低背最长肌中SREBP-1和SCD mRNA的表达量；罗格列酮可以提高背最长肌中PPAR-γ、SREBP-1、FAS、LPL和SCD mRNA的表达量，提高皮下脂肪中SREBP-1和FAS mRNA的表达量。 |
| 关键词: 绵羊 硬脂酰辅酶A去饱和酶 梧桐籽油 罗格列酮 基因表达 |
| DOI：10.11841/j.issn.1007-4333.2018.10.09 |
| 投稿时间：2017-12-20 |
| 基金项目:国家自然科学基金项目（31360560） |
|
| Effects of phoenix tree seed oil and rosiglitazone on the expression of enzymes related to lipid metabolism of sheep tissues |
|
SHI Xiaoxue1, NIU Zhanyu1, XING Chao1, ZHANG Sanrun2, YAN Wanshu1, ZHANG Runhou1
|
| (1.College of Animal Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China;2.Department of Basic Education, Inner Mongolia Medical University, Hohhot 010110, China) |
| Abstract: |
| The objective of this study was to investigate the effects of dietary supplementation of SCD inhibitor (phoenix tree seed oil) and accelerator (rosiglitazone) on the key lipid metabolism regulator and related enzyme's mRNA expressions in the longissimus muscle and subcutaneous adipose tissues of fattening sheep. Eighteen crossbreed ram (Merino sheep♂×Thin-tail Han sheep♀), which were similar in body weight[(27.71±2.64) kg] and body condition, were randomly divided into 3 groups with 6 sheep in each group. The sheep in control group (C group) were fed a basal diet + 4.8% linseeds; those in phoenix tree seed oil group (W group) were fed the C group diets supplemented with 15 g/d phoenix tree seed oil and those in rosiglitazone group (L group) were fed the C group diets supplemented with 8 mg/d rosiglitazone. The trial period lasted 50 d including 10 d of transition period, 5 d of pretrial period and 35 d of trial period. The results showed that:1) Compared to animals in control diet, the expression of PPAR-γ mRNA in the longissimus muscle and subcutaneous adipose of animals in W group were increased by 42% and 28%, respectively. The expression of SREBP-1 and SCD mRNA in the longissimus muscle were decreased by 20% and 24% respectively(P<0.05); 2) Compared to animals in control diet, The mRNA expression levels of PPAR-γ and SREBP-1 in the longissimus muscle of animals in L group were significantly increased by 93% and 35%, respectively (P<0.05). The expression of FAS, LPL and SCD mRNA were increased 36%, 56% and 22%, respectively (P<0.05). and the expression of PPAR-γ and FAS mRNA in subcutaneous adipose of animals in L group were significantly increased by 25% and 32%, respectively (P<0.05). These results proved that the phoenix tree seed oil increased the expression of PPAR-γ mRNA in longissimus muscle and subcutaneous adipose, and decreased the expression of SREBP-1 and SCD mRNA in longissimus muscle. The rosiglitazone increased the expression of PPAR-γ,SREBP-1,FAS,LPL and SCD mRNA in longissimus muscle and the expression of SREBP-1 and FAS mRNA in subcutaneous adipose. |
| Key words: sheep stearoyl-CoA desaturase phoenix tree seed oil rosiglitazone gene expression |
