| 摘要: |
| 为探究准确高效鉴别种子活力的新技术，利用电子鼻系统对未老化（CK）、58℃热水浴老化（Hot water aging method，HW）和50%甲醇老化（Methanol solution aging method，MS）后的山农27小麦种子进行检测，采用主成分分析（Principal component analysis，PCA）、线性判别分析（Linear discriminant analysis，LDA）及载荷分析（Loadings）对老化处理后不同活力水平的小麦种子进行鉴别研究。试验结果：1）老化处理后不同活力水平的小麦种子在电子鼻传感器上有不同的特征性响应图谱；2）PCA分析无法区分热水浴老化后活力接近的小麦种子；3）LDA分析对热水浴老化和甲醇老化不同活力水平的小麦种子均具有较好的区分效果；4）载荷分析确定了热水浴老化各活力水平小麦种子的差异气味主要来自于无机硫化物（W1W）、小分子氮氧化合物（W5S）和有机硫化物（W2W），短链烷烃（W1S）、醇醚醛酮类气体（W2S）和氢气类（W6S）。甲醇老化各活力水平小麦种子的差异气味主要来自于有机硫化物（W2W）、小分子氮氧化合物（W5S）、短链烷烃（W1S）、醇醚醛酮类气体（W2S）和无机硫化物（W1W）。本研究结果揭示以电子鼻技术作为检测手段，通过对小麦种子挥发性成分的检测结合LDA分析，可以实现对不同活力小麦种子进行快速准确高效地鉴别，再结合Loading分析，可以进一步分析相关的差异性气体成分。 |
| 关键词: 电子鼻 小麦种子 活力 主成分分析 线性判别分析 载荷分析 |
| DOI：10.11841/j.issn.1007-4333.2018.09.15 |
| 投稿时间：2017-09-18 |
| 基金项目:科技部十三五国家重点研发计（2018YFD010141）；划公益性行业（农业）科研专项项目（201303002） |
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| Determination of wheat seeds vigor based on electronic nose |
|
ZHANG Tingting1, ZHAO Bin1, YANG Liming2, WANG Jianhua1, SUN Qun1
|
| (1.College of Agronomy and Biotechnology/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China;2.College of Science, China Agricultural University, Beijing 100083, China) |
| Abstract: |
| To investigate novel accurate and effective detection technique for the identification of seed vigor, the wheat seeds of Shannong 27, which were treated by 58℃ hot water aging method (HW) and 50% methanol solution aging method (MS), were analyzed by electronic nose (E-nose). And the data were then processed by principal component analysis (PCA), linear discrimination analysis (LDA) and Loadings. The E-nose sensor data showed that the wheat seeds with different vigor after aging treatment had different characteristic maps. The PCA analysis could not distinguish seeds with similar vigor after HW aging. In contrast to the PCA analysis, the LDA analysis could well distinguish seeds with different vigor after the two aging method. The Loadings analysis demonstrated that there were sulfur compounds (W1W), nitrogen oxides (W5S), aromatic compounds (W2W), methane (W1S), alcohols (W2S) and hydrogen (W6S) differences in the odor between the seeds after HW aging. After MS aging, aromatic compounds (W2W), nitrogen oxides (W5S), methane (W1S), alcohols (W2S) and sulfur compounds (W1W) were the main gas compositions in seeds. In conclusion, our research indicated that E-nose technology could be used as a feasible and reliable method for the determination of wheat seed vigor by volatile constituent analysis and LDA analysis, and different gas components can be further confirmed by Loadings analysis. |
| Key words: electronic nose wheat seeds vigor PCA LDA Loadings |
