| 本文已被：浏览 875次 下载 400次 |
码上扫一扫！ |
| 叶用芥菜小孢子培养技术体系的完善及DH系创制 |
|
邓英1,2, 唐兵1,2, 付文苑1,2, 杨静1,2, 陶莲1,2, 吴康云1,3
|
|
|
| (1.贵州省农业科学院 园艺研究所, 贵阳 550006;2.贵州省园艺工程技术研究中心, 贵阳 550006;3.贵州省农业科学院 辣椒研究所, 贵阳 550006) |
|
| 摘要: |
| 以3个优良的地方品种（杂合体）为试材进行游离小孢子培养，对胚诱导、植株再生、倍性分析和单倍体加倍技术进行研究，完善叶用芥菜小孢子培养技术体系，创制优良DH系应用于育种实践中。在小孢子培养中，选取花蕾是小孢子培养成功的关键，花瓣长/花药长为3/4，花药为黄绿色可作为叶用芥菜小孢子培养选取花蕾的形态指标；供试3个品种都获得胚状体，但品种间出胚差异较大，MR-1共45个胚/皿，MR-2共198个胚/皿，MR-3共4个胚/皿；子叶形胚接种于再生培养基上成株率达98%，获得128株再生植株，利用流式细胞仪鉴定出105株双单倍体，自然加倍率为82%，在组培苗期用0.1%的秋水仙素浸泡单倍体植株茎尖1 h，加倍率为75%。从DH群体中筛选出4个优良DH系，通过品种比较试验鉴选出1个耐抽薹DH系，4月上旬开始抽薹开花，叶柄宽大肥厚，产量高，适合鲜食加工，可补充当地春淡市场。 |
| 关键词: 芥菜 小孢子培养 技术体系 DH系 |
| DOI：10.11841/j.issn.1007-4333.2018.09.07 |
| 投稿时间：2017-06-12 |
| 基金项目:黔农科院自主创新科研专项（[2014]016）；黔科合平台人才（[2016]5671）；贵州省蔬菜产业技术体系建设子项（GZCYX2018）；黔科合平台人才（[2017]5715）；黔农科院青年基金[2018]04号 |
|
| Opertimization of the isolated microspore culture system of leaf mustard and double haploid line producing |
|
DENG Ying1,2, TANG Bing1,2, FU Wenyuan1,2, YANG Jing1,2, TAO Lian1,2, WU Kangyun1,3
|
| (1.Horticultural Research Institute, Guizhou Academy of Agriculturas Sciences, Guiyang 550006, China;2.Guizhou Center for Horticultural Engineering Technology, Guiyang 550006, China;3.Capsicum Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China) |
| Abstract: |
| The microspores of 3 leaf mustard genotypes (MR-1, MR-2 and MR-3) were cultured to research embryos induction, plant regeneration, ploidy analysis, haploid doubling, optimize the isolated microspore culture technical system of leaf mustard and create excellent DH lines of leaf mustard for production practice. Buds selection was crucial to microspore culture. The morphological index of buds were:Petal length:anther length was 3:4; its color was yellow green. Embryos were obtained for the 3 cultivars. There were differences of the number of embryos generating between varieties and MR-1, MR-2 and MR-3 were 45, 198 and 4 per dish, respectively. The natural doubling rate of leaf mustard chromosomes was 82% by flow cytometry and the haploid doubling rate was 75% with 0.1% colchicine soaking stem tip 1 hour in tissue culture seedling. Cotyledon-stage embryos were cultivated on regeneration culture and 128 plants were obtained and the rate of plant formation reached 98%. A total of 105 double haploids were identified by flow cytometer and 4 excellent DH lines were screened from them. A double haploid line was supplied to the spring market for postponed bolting date, thick petioles and high yield and it was suitable for fresh food processing. |
| Key words: mustard microspore culture technical system double haploid line |
