| 摘要: |
| 为探讨葡萄高效再生体系的优化，以黑香蕉葡萄品种叶片、叶柄、茎尖分生组织为外植体，对其再生体系进行了优化，同时比较了不同器官再生的效果。结果显示：适合叶片再生的培养基为：MS+2.0 mg/L 6-BA+0.1 mg/L IBA，但再生率仅为4.88%；适合叶柄再生的培养基为：NN69+1.33 mg/L6-BA+0.02 mg/L NAA，再生率为16.92%。说明叶柄的再生率高于叶片但两者都没有超过20%。并且NAA对黑香蕉叶柄的再生效果好于IBA。NN69基本培养基要优于MS。以茎尖为外植体在起始培养基MS＋6-BA 1.40 mg/L＋NAA 0.09 mg/L和诱导培养基培养MS＋2 mg/L 6-BA＋0.09 mg/L NAA上通过反复损伤芽尖得到茎尖分生组织（Meristematic bulk，MB），其不定芽诱导率高达87.63%。说明利用茎尖分生组织建立的葡萄再生体系更适合葡萄的遗传转化。 |
| 关键词: 葡萄 器官发生 培养基 外植体 分生体 |
| DOI：10.11841/j.issn.1007-4333.2018.09.05 |
| 投稿时间：2017-08-03 |
| 基金项目:国家自然科学基金项目（31471842）；国家重点研发计划（SQ2018YFD020082） |
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| Optimization of Vitis vinifera×V. labrusca ‘Hei Xiangjiao’ adventitious buds regeneration via organogenesis |
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CHANG Xin, LIANG Jinjun, LIU Zhenhua, XIAO Kun, WANG Yan, CUI Haiyang, HU Jianfang
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| (College of Horticulture, China Agricultrual University, Beijing 100093, China) |
| Abstract: |
| To optimize the regeneration system of grape, the regeneration system of Vitis vinifera×V.labrusca ‘Hei Xiangjiao’ leaf, petiole and shoot apical meristem were optimized, and the regeneration effects of different organs were compared. The results showed that:The optimum media for the regeneration of adventitious buds from leaf is MS +2.0 mg/L 6-BA + 0.1 mg/L IBA, but the regeneration rate is only 4.88%; The suitable medium for petiole is NN69 + 1.33 mg/L 6-BA+ 0.02 mg/L NAA, the regeneration rate was increased to 16.92%; The regeneration rate of petiole was higher than that of leaf, but neither exceeded 20%; The effects of NAA enhanced the regeneration of adventitious buds from petioles better than that of IBA; The NN69 medium was better than MS, and the regenerated rate at 40 days was maximum; The stem was cultured on initiation media (MS+6-BA 1.40mg/L+NAA 0.09 mg/L) and then subcultured on induction medium(MS + 6-BA 2mg/L + NAA 0.09 mg/L) and wounding treatments was supplied to produced meristematic bulk tissue. The adventitious bud induction rate is 87.63%. The results indicated that the regeneration system of meristematic bulk was more suitable for grape genetic transformation. |
| Key words: grape organogenesis media explant meristematic bulk |
