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玉米单倍体雌穗育性自然恢复研究
任姣姣1,2, 吴鹏昊1,2, 田小龙1, 陈琛1, 陈绍江1
0
(1.中国农业大学 农学院/国家玉米改良中心/北京市作物遗传改良重点实验室, 北京 100193;2.新疆农业大学 农学院, 乌鲁木齐 830052)
摘要:
为了对玉米单倍体雌穗育性恢复能力进行系统遗传分析,本研究以22个玉米自交系诱导产生的单倍体为材料对雌穗自然恢复能力进行观察,通过分类比较的方法分析单倍体雌穗平均结实株率、平均结实籽粒数和2个雌穗育性恢复指标自然恢复的遗传特点。结果表明,不同自交系的单倍体雌穗平均结实株率为89.54%,变异范围为73.56%~99.18%;单倍体雌穗平均结实籽粒数为15.4粒,变异范围为2.67~59.82粒。单倍体雌穗结实株率的广义遗传力为0.67,平均结实籽粒数的广义遗传力为0.86。22个自交系材料中,B73的单倍体雌穗育性恢复能力较其他试验材料强,单倍体雌穗平均结实株率为99.18%,平均结实籽粒数为59.82粒,基因型在单倍体雌穗育性自然恢复过程中起主要作用。通过对单倍体雌穗育性恢复与雄穗育性恢复性状进行相关性分析发现单倍体雌穗育性恢复与雄穗育性恢复是2个相对独立的过程。
关键词:  玉米单倍体  自交系  自然加倍  雌穗育性恢复  雄穗育性恢复
DOI:10.11841/j.issn.1007-4333.2018.08.01
投稿时间:2017-08-25
基金项目:国家自然科学基金项目(31560392)
Study on the haploid female fertility (HFF) of maize inbred lines
REN Jiaojiao1,2, WU Penghao1,2, TIAN Xiaolong1, CHEN Chen1, CHEN Shaojiang1
(1.College of Agronomy and Biotechnology/National Maize Improvement Center of China/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193;2.College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China)
Abstract:
Haploids induced from 22 inbred lines were pollinated with normal diploid pollen to investigate the genetic inheritance of haploid female fertility (HFF).The average haploid female fertility rate (HFFR)was about 89.54%,and the range was from 73.56% to 99.18%.The average kernel number (AKN) was 15.40 ranging from 2.67 to 59.82.The heritability for HFFR and AKN were 0.67 and 0.86,respectively.It is found that B73 had the best HFF 99.18% and the AKN was 59.82.It is also found that genetic background was the main factor effecting haploid male fertility.No significant correlation was found between haploid male fertility (HMF) and HFF,which reveals that the process of HMF and HFF may have separate processes.
Key words:  maize haploid  inbred line  spontaneous doubling  haploid female fertility  haploid male fertility