| 摘要: |
| 为了解新疆乌鲁木齐市宠物源沙门氏菌耐药及耐药基因携带情况，对分离的39株宠物源沙门氏菌采用琼脂稀释法进行药敏试验，PCR方法进行PMQR因子、β-内酰胺酶基因、氨基糖苷类基因、四环素类基因、酰胺醇类基因及多肽类基因检测。结果表明：乌鲁木齐市宠物源沙门氏菌对环丙沙星和阿莫西林的耐药率均高达97.4%（38/39），对诺氟沙星、恩诺沙星、氨苄西林、卡那霉素、四环素和氟苯尼考的耐药率均高达100%（39/39），对头孢噻呋、安普霉素、阿米卡星和庆大霉素100%敏感；耐药菌株以8耐为主，其主要耐药谱型为CIP-NOF-ENR-AML-AMP-KANA-TE-FFC；主要检出的β-内酰胺酶基因有blaTEM和blaOXA；主要检出的PMQR因子有qnrS、oqxA、oqxB和aac（6'）-Ib-cr；主要检出的氨基糖苷类基因有aadA2和ant（3"）-Ia；主要检出的四环素类基因有tetB；主要检出的酰胺醇类基因有floR。耐药基因以blaTEM+blaOXA+qnrS+oqxA+oqxB+aac（6'）-Ib-cr+aadA2+ant（3"）-Ia+tetB+floR共存类型为主。因此临床治疗宠物疾病时应尽量少用人用药物特别是人用新药，多使用兽用敏感药物，避免人发病时无药可用事件的发生。 |
| 关键词: 宠物 沙门氏菌 耐药性 耐药基因 检测 |
| DOI：10.11841/j.issn.1007-4333.2018.07.09 |
| 投稿时间：2017-10-05 |
| 基金项目:国家自然科学基金-新疆联合基金项目（U1503185） |
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| Detection of drug resistance and resistant genes of Salmonella from pets in Urumqi,Xinjiang |
|
LIN Yajun, GUO Fei, XIA Lining, YAO Xiaohui
|
| (College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China) |
| Abstract: |
| In order to investigate the resistance to antibacterial drugs and the drug-resistant genes of Salmonella isolates from pets in Urumqi,Xinjiang.Thirty-nine Salmonella isolates' antimicrobial susceptibility were tested by agar dilution method.PCR method was used to detect the resistance genes of PMQR,β-lactamase,aminoglycoside,tetracycline,amide alcohol and peptide.The results showed that:The isolates were 97.4% (38/39) resistant to ciprofloxacin and amoxicillin;The isolates were 100% resistant to norfloxacin,enrofloxacin,ampicillin,kanamycin,tetracycline and florfenicol;The isolates were 100% sensitive to ceftiofur,apramycin,amikacin and gentamicin.The main resistance profile was CIP-NOF-ENR-AML-AMP-KANA-TE-FFC.The blaTEM and blaOXA were the mainly detected genes of beta lactamases.The qnrS,oqxA,oqxB and aac (6')-Ib-cr were the mainly detected factors of PMQR.The aadA2 and ant (3")-Ia were the mainly detected genes of aminoglycoside.The tetB and floR were mainly detected genes of tetracycline and amide alcohol.The coexistence of multiple genes was blaTEM+blaOXA+qnrS+oqxA+oqxB+aac (6')-Ib-cr+aadA2+ant(3")-Ia+tetB+floR. Therefore,to avoid no drug available for treatment human illness situation occurs in future,animal specific sensitive medicines should be used to in pets not drugs for human,especially not new drugs. |
| Key words: pet Salmonella resistance resistance genes detection |
