| 摘要: |
| 为更好地利用国外引进的杏（Armeniaca vulgaris Lam.）种质资源，以北寨红杏（A.vulgaris ‘Beizhaihongxing’）和金太阳杏（A.vulgaris ‘Goldensun’）为对照，对从匈牙利引进的优质杏品系Hybrid18/79（A.vulgaris ‘18/79’）和Hybrid10/10（A.vulgaris ‘10/10’）的有效花率、自然座果率和自交座果率以及花粉管生长等特性进行研究，同时，通过S-allele-specific PCR扩增程序进行S基因型的分析测定。结果表明：Hybrid18/79、Hybrid10/10和北寨红杏的自交坐果率分别为18.05%，2.27%和1.68%，Hybrid18/79为自交亲和性品系，北寨红杏和Hybrid10/10为自交不亲和品种和品系。与自交亲和的金太阳杏相比，Hybrid10/10自花授粉后花粉管在花柱中生长受到阻塞，至花柱2/3处，花粉管顶端膨大，停止生长，而金太阳杏可以顺利进入胚囊进行受精。Hybrid18/79、Hybrid10/10和北寨红杏的S基因型分别为Su1Su2、S16S16和S30Su1 ，序列比对发现Hybrid18/79的2个S基因（Su1、Su2）都是新发现的S基因，北寨红杏的一个S基因（Su1）与Hybrid18/79中的一个S基因（Su1）一样，也为新发现的S基因。目前已在GenBank进行了登录，序列登录号为MF685202和MF685203。 |
| 关键词: 杏品系 自交不亲和 S基因型 匈牙利 |
| DOI：10.11841/j.issn.1007-4333.2018.07.05 |
| 投稿时间：2017-09-15 |
| 基金项目:农业部引进国际先进农业科学技术计划（948计划）项目（2010-Z14）；农业部（2015-2019）落叶果树遗传与分子育种创新团队项目；科技部政府间科技合作项目（国科外字2013-83）；中国农业大学-新疆科研院校合作基金项目（2017TC011）；国家自然科学基金项目（31661143046） |
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| Self-compatibility analysis of two high quality apricot strains introduced from Hungary |
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MA Yingzheng, WANG Shufang, NI Xiaomin, WANG Danni, HU Jiayi, ZHAO Yang, ZHANG Lusheng
|
| (College of Horticulture, China Agricultural University, Beijing 100193, China) |
| Abstract: |
| In order to make better use of the apricot germplasm resources introduced from Hungary,this study was focused on the effective flower rate,natural fruit setting rate,self-setting fruit rate,pollen tube growth and other characteristics of two high quality apricot strain Hybrid18/79 and Hybrid10/10 introduced from Hungary.Beizhaihongxing and Golden Sun apricot were used as control.The S genotype was assayed and determined by S-allele-specific PCR.The results showed that:The self-pollination fruit setting rates of Hybrid18/79,Hybrid10/10 and Beizhaihongxing were 18.05%,2.27% and 1.68% respectively.Hybrid18/79 was a self-compatibility strain.Both Beizhaihongxing and Hybrid10/10 were self-incompatibility cultivars and strains.After self-pollinating,the pollen tube growth of Hybrid 10/10 was blocked in style,and the pollen tube expanded to the top and stopped growing.In comparison,the tube of the self-compatibility cultivar Golden Sun apricot had no growth blocking,which could successfully enter embryo sac for fertilization.The S-genotypes of Hybrid18/79,Hybrid10/10 and Beizhaihongxing were identified as Su1Su2, S16S16 and S30Su1 respectively.The results of sequence alignment showed that Su1 and Su2 were new genes.One S gene (Su1) of Beizhaihongxing apricot was the same as the Su1 in Hybrid18/79.The two discovered genes had been registered in GenBank with accession numbers MF685202 and MF685203. |
| Key words: apricotgermplasm self-incompatibility S genotype Hungary |
