| 摘要: |
| 为探索驯鹿（Rangifer tarandus）Morf4l1基因的生物学特性以及雌、雄驯鹿茸顶端表皮组织表达差异，揭示Morf4l1对鹿茸生长的重要生物学意义，采用RT-PCR技术和分子克隆技术成功获得驯鹿Morf4l1基因cDNA，以驯鹿Morf4l1基因cDNA为依据，展开生物信息分析和雌、雄驯鹿鹿茸表皮组织中表达差异的研究。结果表明：1）驯鹿Morf4l1基因CDS区全长为972 bp，共编码323个氨基酸。2）驯鹿Morf4l1蛋白是一种相对分子量为37.20 ku的不具跨膜结构的亲水性蛋白质，其二级结构主要以β转角为主。3）驯鹿Morf4l1编码蛋白氨基酸序列与其他18个物种该蛋白氨基酸序列发现相似度介于85%~100%，其中与白尾鹿相似度最高为100%，与原鸡相似度最低为85%，与其他亲缘关系较近物种（如牛、羊）的相似度能达到99%；结合系统进化树发现驯鹿Morf4l1基因在进化中高度保守，各物种之间的遗传距离不超过0.035。4）荧光定量结果显示雌性驯鹿茸顶端表皮组织Morf4l1基因相对表达量是雄性的2.127±0.032倍。本研究发现驯鹿Morf4l1基因在进化中高度保守，是鹿茸生长发育的关键基因，其在驯鹿鹿茸表皮组织中相对表达量受性别影响较为明显。 |
| 关键词: 驯鹿 Morf4l1基因 cDNA克隆 生物信息分析 基因表达 |
| DOI：10.11841/j.issn.1007-4333.2018.06.09 |
| 投稿时间：2017-10-13 |
| 基金项目:国家林业局珍稀濒危物种野外救护与繁育项目（2012）；中央高校基本科研业务专项资金项目（2572016AA42） |
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| cDNA cloning and expression analysis of Morf4l1 gene in the antler top of female and male reindeer |
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WANG Qianghui, ZHAI Jiancheng, XIA Yanling, LI Heping
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| (College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China) |
| Abstract: |
| In order to explore the biological characteristics and expression of Morf4l1 gene in reindeer.RT-PCR and molecular cloning were used to obtain the cDNA of Morf4l1 .Based on the information of Morf4l1 cDNA, a series of bioinformatics analysis was carried out.The results showed that:1) The full length of reindeer Morf4l1 CDS of was 972 bp, which encoded 323 amino acids.2) Morf4l1 protein was a hydrophilic protein of 37.20 ku without transmembrane structure, and its secondary structure was mainly β -sheet.3) The amino acids sequence of Morf41 protein was compared with that of 18 other species, the overall similarity was ranged 85% to 100%.The highest was white tail deer of 100% and the lowest was Raw chicken of 85%.Other species such as cattle, sheep with reindeer close relatives, their highest similarity reached 99%;Through the phylogenetic tree analysis, it was found that reindeer Morf411 was highly conserved during the evolution, and the genetic distances among species were not more than 0.035.4) The fluorescence quantitative analysis showed that expression of Morf4l1 gene in the epidermis of female reindeer top antler was 2.127±0.032 times more than in the male.In conclusion, reindeer Morf4l1 gene was highly conserved during evolution and was an important for antler development.Its expression was affected by sex of reindeer. |
| Key words: reindeer Morf4l1 gene cloning of cDNA bioinformatics analysis gene expression |
