| 摘要: |
| 通过变色圈法和透明圈法从泥土中筛选出脂肪酶产生菌，并根据菌株的菌落形态、革兰氏染色和16S rDNA序列分析确定筛选得到菌株的种属。采用罗丹明B （Rhodamine B）平板初筛出51株脂肪酶产生菌，以荧光圈直径或透明圈直径与菌落直径的比值（HC）为指标，选取HC>1.60的9株脂肪酶产生菌，采用橄榄油乳化液平板进行复筛，对HC进行测量比较筛选出1株高产脂肪酶的菌株L-17，其HC为2.27。利用透明圈法对菌株L-17的粗酶液的酶活力进行测定，HC为3.07，表明菌株L-17具有较高的酶活力。菌株L-17经初步鉴定为洋葱伯克霍尔德菌（Burkholderia cepacia），为一株高产脂肪酶的菌株，具有潜在的研究和开发价值。 |
| 关键词: 脂肪酶 筛选 鉴定 洋葱伯克霍尔德菌 |
| DOI：10.11841/j.issn.1007-4333.2018.05.010 |
| 投稿时间：2017-07-07 |
| 基金项目:现代农业产业体系北京市创新团队建设-鲟鱼、鲑鳟鱼（SCGWZJ201711） |
|
| Screening of high lipase-producing strains |
|
XIE Qing, WANG Yao, CHEN Shangwu, LI Pinglan
|
| (College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) |
| Abstract: |
| Lipase-producing strains isolated from soil were screened by the color-changing circle method and transparent circle method.These strains were identified according to their morphology,Gram staining and 16S rDNA sequence analysis.A total of 51 high lipase-producing strains were isolated on Rhodamine B screening plates by using HC index.Compared the HC of the strains screened by olive oil screening plates,L-17,the strain of highest HC 2.27,was identified as target strain.The enzyme activity of L-17 was determined by transparent circle method,and its HC was 3.07 which proved the high enzyme activity of L-17.The L-17 strain was identified as Burkholderia cepacia with high lipase yield,which had potential research and development value. |
| Key words: lipase screening identification Burkholderia cepacia |
