| 摘要: |
| 为研究日粮中添加硬脂酰辅酶A去饱和酶（SCD）的活性促进剂（罗格列酮）和抑制剂（梧桐子油）对绵羊背最长肌及皮下脂肪组织中的脂肪酸合成酶（FAS）、脂蛋白脂酶（LPL）、乙酰辅酶A羧化酶（ACC）以及SCD酶活性的影响，选18只体重为27.71±2.64 kg、生理状态相似的杂交公羊（美利奴♂×小尾寒羊♀），将其随机分为3组，每组6只，单栏饲养。对照组饲喂基础日粮，梧桐子油组饲喂基础日粮+15 g/d梧桐子油，罗格列酮组（L组）饲喂基础日粮+8 mg/d罗格列酮。结果表明：1）与对照组相比，罗格列酮可以显著提高绵羊背最长肌中LPL、SCD和皮下脂肪中FAS酶的活性（P<0.05），然而，对背最长肌和皮下脂肪中ACC酶的活性没有影响。2）与对照组相比，梧桐子油抑制了绵羊背最长肌中ACC和SCD酶的活性，但对皮下脂肪中的ACC、FAS、LPL和SCD酶活性没有影响。本试验表明罗格列酮可以通过增强机体对胰岛素的敏感性，进而提高SCD和FAS的活性，另外罗格列酮还可以通过激活PPAR-γ，进而提高组织中LPL的活性；富含不饱和脂肪酸的梧桐子油可以通过抑制ACC的基因表达而影响组织中其酶活性的变化，SCD的活性受到梧桐子油中苹婆酸和其他多不饱和脂肪酸的抑制作用而表现出活性降低的结果。 |
| 关键词: 硬脂酰辅酶A去饱和酶 脂肪酸合成酶 脂蛋白脂酶 乙酰辅酶A羧化酶 梧桐子油 罗格列酮 酶活 |
| DOI：10.11841/j.issn.1007-4333.2018.05.007 |
| 投稿时间：2017-09-10 |
| 基金项目:国家自然科学基金项目（31360560） |
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| Effects of dietary supplementation of rosiglitazone and phoenix tree seed oil on the activities of lipase-related enzymes in sheep |
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SHI Xiaoxue1, NIU Zhanyu1, XING Chao1, ZHANG Sanrun2, YAN Wanshu1, ZHANG Runhou1
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| (1.College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;2.Department of Basic Education, Inner Mongolia Medical University, Hohhot 010110, China) |
| Abstract: |
| The objectives of this study were to investigate the effects of dietary supplementation of the SCD accelerator (rosiglitazone) and inhibitor (phoenix tree seed oil) on the activities of FAS,LPL,ACC and SCD in longissimus muscle and subcutaneous adipose tissues in fattening sheep.Eighteen crossbreed ram (Merino sheep♂×Han sheep♀),similar in body weight (27.71 + 2.64 kg) and birth date,were randomly divided into three groups with six animal each:Animals in the first group (C group) were fed with control diet;Animals in the second group (L group) were fed with control diet plus rosiglitazone at the rate of 8 mg/d;the third group (W group) were fed with control diet plus phoenix tree seed oil at the rate of 15 g/d.The results showed that:1) Compared to animals in control diet,animals in L group had significantly higher LPL and SCD activities in longissimus muscle and significantly higher FAS activity in subcutaneous fat (P<0.05) in sheep while no significant effects were observed on the ACC activity in the longissimus muscle and subcutaneous fat.2) Compared to animals in control diet,animals in W group had lower ACC and SCD activities in longissimus muscle,but no significant effects on the activities of ACC,FAS,LPL and SCD in the subcutaneous fat were observed.This study showed that rosiglitazone can increase the activities of SCD and FAS by improving body sensitivity to insulin In addition,rosiglitazone can also enhance the activity of LPL in tissue by activating PPAR-γ,The phoenix tree seed oil,which is rich in unsaturated fatty acids,can affect enzyme activity in the tissues by inhibiting the expression of ACC gene,the activity of SCD was reduced by sterculic acid and other polyunsaturated fatty acids in phoenix tree seed oil. |
| Key words: stearyl CoA desaturase fatty acid synthase lipoprotein lipase acetyl-CoA carboxylase phoenix tree seed oil rosiglitazone enzyme activity |
