| 本文已被：浏览 890次 下载 354次 |
码上扫一扫！ |
| 544份水稻种质稻瘟病抗性鉴定及抗性基因的分布研究 |
|
李刚1, 袁彩勇1, 曹奎荣2, 孙祥良2, 李军2, 王健1, 程保山1, 罗伯祥1, 徐卫军1, 唐九友3, 储成才3
|
|
|
| (1.江苏徐淮地区淮阴农业科学研究所/淮安市农业生物技术重点实验室, 江苏 淮安 223001;2.嘉兴市农业科学研究院(所), 浙江 嘉兴 314016;3.中国科学院 遗传与发育生物学研究所/植物基因组学国家重点实验室, 北京 100101) |
|
| 摘要: |
| 采用苗期喷雾接种鉴定方法并结合10个主效抗性基因的特异性分子标记检测，分析544份水稻种质资源的稻瘟病抗性水平及其携带的抗性基因分布情况。结果表明：稻瘟病抗性水平达高抗、抗、中抗、中感、感及高感的材料分别为25、50、78、152、156和83份；10个分子标记对应的抗病基因在供试材料中均被检测到，含有2和3个抗性基因的材料分别为4和17份（占0.7%和3.1%），476份材料含有4~6个抗性基因（占87.5%），47份材料含有7个抗性基因（占8.6%）；品种抗病反应与其抗病基因种类密切相关，Pi5、Pita、Pi9、Pib等4个基因对6个强致病鉴定小种抗性表现较好。隆粳968、秀水134、嘉58、津稻263、淮稻20号、盐稻10号、谷梅4号等品种含有多个主效抗病基因，连续多年达到高抗水平。利用分子标记辅助选择将不同来源的主效抗病基因聚合到同一品种中，是控制该病害最经济有效的途径。 |
| 关键词: 稻瘟病 抗性基因 分子标记 基因聚合 |
| DOI：10.11841/j.issn.1007-4333.2018.05.003 |
| 投稿时间：2017-08-12 |
| 基金项目:国家重点研发计划"七大作物育种"重点专项（2017YFD0100400）；中国科学院战略性先导科技专项（XDA08010401）；淮安市应用研究与科技攻关（农业）计划（HAN2015008、HAN201613）；浙江省科技计划项目（2016C32104） |
|
| Evaluation and distribution of the blast resistance genes of 544 rice materials |
|
LI Gang1, YUAN Caiyong1, CAO Kuirong2, SUN Xiangliang2, LI Jun2, WANG Jian1, CHENG Baoshan1, LUO Boxiang1, XU Weijun1, TANG Jiuyou3, CHU Chengcai3
|
| (1.Huaiyin Institute of Agricultural Sciences in Xuhuai Region of Jiangsu/Huaian Key Laboratory of Agricultural Biotechnology, Huai'an 223001, China;2.Jiaxing Academy of Agricultural Sciences, Jiaxing 314016, China;3.Institute of Genetics and Developmental Biology/State Key Laboratory of Plant Genomics, Chinese Academy of Sciences, Beijing 100101, China) |
| Abstract: |
| A total of 544 rice materials were assessed for blast resistance and resistance genes distribution by inoculation and screening of molecular markers tightly linked to 10 major resistance genes.Among the 544 materials,25,50,78,152,156 and 83 exhibited high resistance,resistance,moderate resistance,moderate susceptibility,susceptibility and high susceptibility,respectively.Ten resistance genes corresponding to markers were found in these materials;Two and three resistance genes were found in 4 and 17 materials (0.7% and 3.1%);Four to six resistance genes were found in 476 materials (87.5%);Seven resistance genes were found in 47 materials (8.6%).The resistance of all rice materials was significantly associated with resistance genes.Pi5,Pita,Pi9 and Pib exhibited high resistance to 6 physiological races.The majority of resistance genes were found in Longjing 968,Xiushui 134,Jia 58,Jindao 263,Huaidao 20,Yandao 10 and Gumei 4.Pyramiding different resistance genes in rice by marker-assisted selection was suggested as the most economical,effective,and environment friendly measure for controlling rice blast disease in this study. |
| Key words: rice blast resistance genes molecular markers gene pyramiding |
