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不同柿种质雄花表型、活性成分及体外抗氧化活性
韩卫娟1,2, 孙鹏1,2, 刁松锋1,2, 索玉静1,2, 张悦1,2, 傅建敏1,2
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(1.国家林业局 泡桐研究开发中心, 郑州 450003;2.中国林业科学研究院 经济林研究开发中心, 郑州 450003)
摘要:
为了解柿雄花表型、活性成分、抗氧化活性及其相关性,以8份不同柿种质雄花为材料,采用分光光度法和高效液相色谱法进行测定。结果表明:表型性状存在丰富的变异,平均变异系数为33.50%,变化幅度为0%~61.37%;黄酮、总酚、单宁和维生素C含量存在显著性差异,变异系数分别为51.65%、40.19%、18.87%和109.10%;清除自由基能力(DPPH·和ABTS+·)、还原能力(Fe3+和Cu2+)及络合能力(Fe2+)显著,与活性成分呈显著的相关关系,与表型相关性不显著;雄花表型间、雄花与维生素C含量正相关关系显著。主成分分析和聚类分析结果表明:20个性状可以缩减为3个主成分,累积方差贡献率达到78.90%,8份资源可聚为4类,在开发利用中可按照具体目标进行选择。
关键词:    雄花  活性成分  抗氧化活性  相关性  主成分分析
DOI:10.11841/j.issn.1007-4333.2018.04.04
投稿时间:2017-06-12
基金项目:中国林业科学研究院重点项目与任务(CAFYBB2017ZA004-3);中国林业科学研究院青年项目配套类(CAFYBB2016QB017)
Phenotypic characteristics,active components and antioxidant activities of the male flowers of different Diospyros materials
HAN Weijuan1,2, SUN Peng1,2, DIAO Songfeng1,2, SUO Yujing1,2, ZHANG Yue1,2, FU Jianmin1,2
(1.Non-timber Forestry Research & Development Center, Chinese Academy of Forestry, Zhengzhou 450003, China;2.Paulownia Research & Development Center of China, State Forestry Administration, Zhengzhou 450003, China)
Abstract:
In order to systematically evaluate the phenotypic characteristics,active components contents,and antioxidant activities of male flowers of different Diospyros materials,8 kinds of materials were measured by spectrophotometric and HPLC methods.The results showed that high degree and great range existed in phenotypic characteristics,with the coefficient of variation ranging from 0% to 61.37%,with 54.95% on average.There were significant differences in flavonoids,polyphenols,tannin and vitamin C contents,with the coefficient of 51.65%、40.19%、18.87% and 109.10%,respectively.The antioxidant activities including DPPH·,ABTS+·scavenging ability,Fe3+ and Cu2+ reducing ability,the chelating ability of Fe2+ and Cu2+ was excellent,and they were significantly correlated with active components and no significant correlation with phenotypic characteristics.According to principal component and cluster analysis,three principal components with the cumulative contribution rates reaching 78.900% were obtained from twenty tested indexes.In addition,the eight materials were divided into four groups and selections should be made according to different targets for development and utilization in the further.
Key words:  male flower  Diospyros  active components  antioxidant activities  correlation  principal component analysis