| 摘要: |
| 为了解超广谱β-内酰胺酶（ESBLs）及其相关耐药基因在大熊猫粪便源和环境源大肠杆菌的流行状况，采用双纸片和K-B纸片扩散法对分离自圈养大熊猫粪便（n=96株）和生活环境（n=29株）的大肠杆菌菌株，进行了产ESBLs菌株筛选和耐药性检测，并采用PCR及测序法检测菌株ESBLs耐药基因型。结果表明：96株粪便源和29株环境源菌株ESBLs检出率分别为26.04%和24.14%；ESBL阳性菌对多数药物耐药率高于阴性菌（P ≤ 0.05）；不同来源菌株对氨苄西林等5种药物呈较高水平耐药性（耐药率≥ 30%），且呈严重的多重耐药性；粪便源菌株blaTEM、blaCTX-M、blaOXA和blaSHV基因检出率分别为16.67%、11.46%、7.29%和0%，而环境源菌株以上基因检出率分别为13.79%、10.34%、0%和0%。研究发现，产ESBLs菌株较广泛存在于大熊猫粪便源和环境源大肠杆菌中，这些菌株具有更严重和复杂的耐药表型，其ESBLs基因型主要为blaTEM和blaCTX-M。 |
| 关键词: 大肠杆菌 耐药性 β-内酰胺酶 基因型 |
| DOI：10.11841/j.issn.1007-4333.2018.03.09 |
| 投稿时间：2017-04-18 |
| 基金项目:国家科技部支撑计划项目（2012BAC01B06） |
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| Detection of plasmid-mediated β-lactams resistance genes in Escherichia coli isolates from feces of captive Giant Panda and their habitats |
|
QIN Zhenbin1, HOU Rong2, LIN Juchun1, GAO Tongtong1, LIU Songrui2
|
| (1.College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;2.Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China) |
| Abstract: |
| To investigate the prevalence and types of extended-spectrum β -lactamases (ESBLs) resistance genes in Escherichia coli from feces and habitats of captive Giant Panda,double disc diffusion test and K-B disk diffusion method were used to confirm ESBLs and antibiotic resistance in E.coli isolates from captive Giant Panda feces (n=96) and their habitats (n=29).Additionally,the ESBLs genes were detected by PCR-sequencing.The results showed that:The detection rates of 96 fecal and 29 habitat strains producing ESBLs were 26.04% and 24.14%,respectively;The resistance rate of ESBLs positive strains was higher than that of negative strains (P ≤ 0.05),and the strains from different resources harbored high resistance levels and multi-resistance to 5 antimicrobial agents including ampicillin (resistance rate ≥ 30%);The prevalence rate of blaTEM,blaCTX,blaOXA and blaSHV in fecal strains were 16.67%,11.46%,7.29% and 0%,respectively.However,the rates of above genes in habitat strains were 13.79%,10.34%,0% and 0%,respectively.In conclusion,ESBLS-producing E.coli exited widely in the strains tested.These strains had more serious resistance and complicated resistance mechanisms.blaTEM and blaCTX are dominant genotypes in these strains from Giant Panda and their habitats. |
| Key words: Escherichia coli resistance extended-spectrum β-lactamases genotype |
