| 摘要: |
| 为筛选出高效降胆固醇乳杆菌，挖掘其潜在益生功能，从民间自制泡菜样品中分离乳杆菌，采用邻苯二甲醛法筛选高效降胆固醇菌株，利用16S rDNA进行分子生物学鉴定，并通过耐酸、耐胆盐及抑菌性实验，测定菌株的益生潜能。结果表明：1）从分离出的11 株乳杆菌中筛选出PL2、PL5、PL16和PL194等4 株高效降解胆固醇菌株，其体外胆固醇降解率分别为48.22%、51.67%、48.43%和48.56%；2）结合菌落特征、部分生理生化试验及16S rDNA 基因序列比对鉴定4 株乳酸菌均为植物乳杆菌（Lactobacillus plantarum）；3）该4 株乳酸菌均可在pH 2.0的环境下生存3 h，菌株PL2和PL5可以在0.3%的胆盐浓度下存活3 h；4）菌株PL5对致病性大肠杆菌、沙门氏菌、金黄色葡萄球菌和福氏志贺氏菌均有显著的抑制作用。总之，植物乳杆菌PL5是具有开发前景的高效降胆固醇益生菌。 |
| 关键词: 植物乳杆菌 降胆固醇 耐酸 耐胆盐 抑菌作用 |
| DOI：10.11841/j.issn.1007-4333.2018.02.05 |
| 投稿时间：2017-02-15 |
| 基金项目:国家重点研发计划（2016YFD0501409-03）；国家自然基金项目（NSFC：31372331）；现代农业产业技术体系建设专项（CARS-38）；公益性行业（农业）科研专项（201303091） |
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| Screening and evaluation of cholesterol-lowering Lactobacillus plantarum strains |
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ZHOU Haizhu1, GAO Yunhang1, GUO Wei2, GOU Changlong1, TAO Dapeng1, LOU Yujie1
|
| (1.Animal Science and Technology College, Jilin Agricultural University, Changchun 1301182, China;2.Animal Husbandry General Station of Jilin Province, Changchun 130062, China) |
| Abstract: |
| The aim of this study was to screen the Lactobacillus with efficient cholesterol-lowering ability and discover its beneficial potency.Eleven Lactobacillus strains were isolated from homemade pickle samples and screened for high cholesterol-lowering by phthalic aldehyde method and 16S rDNA molecular biology identification.The acid tolerance,bile tolerance and antibacterial tests were then conducted to analyze their probiotic properties.The results showed that:1) High cholesterol-lowering strains,PL2,PL5,PL16 and PL19,were obtained from the screen,and their cholesterol degradation rate were 48.22%,51.67%,48.43% and 48.56%,respectively.2) The four strains were identified as L.plantarum by morphological observation and molecular biology identification.3) All strains survived in pH 2.0 for three hours.Strains PL2 and PL5 survived under ambient of 0.3% bile salt concentration for three hours.4) It was found that strain PL5 displayed significant inhibitory effect on four common pathogens.In conclusion,strain PL5 was a potential probiotics with excellent cholesterol-lowering effect. |
| Key words: Lactobacillus plantarum cholesterol-lowering acid tolerance bile salt tolerance antibacterial effect |
