| 摘要: |
| 为探讨蒙药成份复方对乳腺炎模型小鼠免疫功能的影响，96只产后3~5 d泌乳期母鼠被随机分为4组（n=24），灌药3 d后，于第4 d的末次灌药后1 h复制小鼠乳腺炎病理模型，继续用药治疗3 d。并于复制模型前0、48和72 h用碳廓血清法测巨噬细胞吞噬活力；MTT法测自然杀伤细胞（Natural killer cell，NK cell）活性；脱臼处死小鼠称胸腺和脾脏湿重并计算小鼠免疫脏器指数；ELISA法测小鼠血清中白介素-4（IL-4）、干扰素（IFN-γ）含量。结果表明蒙药成份复方可以显著提高小鼠免疫脏器指数、促进单核巨噬细胞吞噬能力和NK细胞杀伤活性、下调IL-4含量、上调IFN-γ的含量。由此可见，蒙药成份复方可以提高乳腺炎模型小鼠非特异性免疫及特异性免疫功能。 |
| 关键词: 蒙药 成份复方 非特异性免疫 特异性免疫 |
| DOI：10.11841/j.issn.1007-4333.2018.01.13 |
| 投稿时间：2017-02-21 |
| 基金项目:国家自然科学基金项目（31260590）；国家科技支撑项目（2015BAD29B06） |
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| Immunoregulation effect of Mongolian medicine composition compounds on mastitis model mice |
|
GAO Ruijuan1, WANG Chunjie1, Aorigele2, JIA Zhifeng1
|
| (1.College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;2.College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China) |
| Abstract: |
| To explore the immune-regulation effect of Mongolian medicine compound prescription on mastitis model mice, a total of 96 postnatal 3-5 d lactating female mice were randomly divided into 4 groups (n=24). Oral medication was conducted on mice for three days and the pathological model of murine mastitis was then reproduced in 1 h after final oral medication at the 4th day. The phagocytic activity of macrophages was measured by broad-width carbon at 0, 48 and 72 h, respectively. MTT assay was adopted to detect NK cell activity. The mice were dislocated and the wet weight of the thymus and spleen measured. Immune organ index was calculated. The levels of IL-4 and IFN-γ in serum were determined by ELISA. Mongolian medicine compound significantly improved immune organ index of mice, promoted the phagocytic capacity of monocyte-macrophage and NK cell killing activity, down-regulated the content of IL-4, increase the content of IFN-γ. In conclusion, Mongolian medicine compound can improve the mastitis model mice nonspecific immune and specific immune function. |
| Key words: Mongolian medicine composition compound nonspecific immune specific immune |
